MagNA Pure 96 System

MagNA Pure 96 System including instrument, kits, and accessories are for in vitro diagnostic use unless otherwise noted.

Effortless purification, confidence from automation

The MagNA Pure 96 System purifies DNA, RNA, and viral nucleic acids from a wide range of starting materials using magnetic glass particle technology.

Preprogrammed software protocols are tailored to a diversity of sample requirements. Resulting nucleic acids are suitable for a broad range of downstream applications.

The MagNA Pure 96 System effortlessly processes up to 96 samples in less than one hour using barcoded, prefilled trays with ready-to-use reagents.

Experience seamless data management using intuitive features, such as the link to a laboratory information management system (LIMS).

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Features	Benefits
Speed and throughput Approximately 1 hour for processing a 96 sample run Multiple sample types can be processed in one run	Quick time-to-result for clinicians and patients
Convenience Only two kits needed to cover various diagnostic applications Few reagents and consumables needed Connectivity (LIMS and bidirectional file sharing)	Minimized error risk Low training effort & hands-on time
Robustness Insusceptible against tested substance interferences Very stable against cross contamination	Confidence & trust in your results
Performance Broad range of sample materials Excellent precision, purification efficiency and linear range Minimal lot-to-lot variation	One solution for various needs
Service and support Globally, approximately 3,500 employees at Roche Diagnostics are dedicated to service and support Roche is a leading supplier of IVD products. No other company has more experience in molecular diagnostics	Minimum downtime Cost effectiveness

Software

The Magna Pure 96 system is on software version 3.1 currently.

If you need assistance to update your software system, please contact your local Roche representative.

Specifications

Number of samples

1 to 96 reactions per run
Sample volume

50 to 500 µL with 96 reactions per run
1000 µL with 48 reactions per run
Maximum number of sample types within one run:

10 (whole blood, plasma, serum, urine, swabs, stool, sputum, BAL, CSF, bacterial cultures)
Run time for 96 reactions:

Approx. 50-60 minutes for 200 µL sample volumes
Approx. 80-90 minutes for 500 µL sample volumes
Reagent type:

Prefilled, ready-to-use
LIMS connectivity

Yes
21CFR part 11 (subsection B) compatibility

Yes
IVD compliance

Instrument complies with the requirements of IVD directive 98/79/EC

The MagNA Pure 96 System is a high-throughput instrument for automated nucleic acid purification. Typical run duration is less than one hour for 96 standard volume samples. Applications are very flexible, due to a diversity of software purification protocols using barcoded, prefilled trays with ready-to-use reagents.

MagNA Pure 96 kits are designed profiting from our more than ten year expertise in magnetic glass particle technology. We have streamlined the purification process to a very small number of reagents, consumables and steps.

Optimized preprogrammed purification protocols ensure routine sample handling and high quality, reproducible nucleic acid yields. Choose the appropriate purification protocol for your sample material and target nudeic acid:

Target	Protocol name	Input sample type and sample volumes The latest version of MagNA Pure 96 protocols are available to download from the table below (not available in US)													Elution volume	Run time*** (h:mm)
Target	Protocol name	Whole blood	Plasma (citrate)	Plasma (EDTA)	Serum	Swabs	BAL*	Sputum	CSF	Urine	Stool	Cultured cells	Fresh-frozen tissue	FFPET**	Elution volume	Run time*** (h:mm)
Bacterial, fungal and viral NA	Pathogen Universal 200 ¹	200 μL													50, 100 μL	1:00
	Viral NA Plasma SV ¹			50, 100, 200 μL												1:00
	Viral NA Plasma ext lys SV ¹ Protocol optimized with external lysis reagent	350, 450 μL lysate from 100, 200 μL sample														1:00
	Viral NA Universal SV ¹	50, 100, 200 μL			50, 100, 200 μL											1:00
	Pathogen Universal 500 ²	500 μL														1:30
	Pathogen Universal 1000 ²	1000 μL														1:40
	Viral NA Plasma LV ²			500 μL												1:10
	Viral NA Plasma LV 1000 ²			1000 μL												1:20
	Viral NA Plasma ext lys LV ² Protocol optimized with external lysis reagent		1000 μL lysate from 500 μL sample													1:10
	Viral NA Universal LV ²		500 μL		500 μL											1:30
	Viral NA Universal LV 1000 ²		1000 μL		1000 μL											1:30
Genomic DNA	DNA Blood SV ¹ Recommended for elution of predominantly double-stranded DNA	50, 100 or 200 μL ≤ 2x10⁶ cells/samples													50, 100 μL	1:00
	DNA Blood ext lys SV ¹ Protocol optimized with external lysis reagent	300, 350, or 450 μL lysate from 50, 100, 200 μL sample													50, 100 μL	0:50
	DNA Cells SV ¹											Up to 5x10⁵ cultured cells in 200 μL PBS			100 μL	1:00
	DNA Tissues SV ¹												Up to 5 mg in 200 μL		100, 200 μL	1:20
	DNA FFPE SV ¹													200 μL lysate from 2x5 μL sections	50, 100 μL	1:00
	DNA Blood LV ²	500 μL (≤ 1x10⁷ cells/ml)													100, 200 μL	1:20
	DNA Blood LV 1000 ²	1000 μL (≤ 1x10⁷ cells/ml)													100, 200 μL	1:30
	DNA Blood ext lys LV ² Protocol optimized with external lysis reagent	1000 μL lysate from 500 μL sample (≤ 1x10⁷ cells/ml)													100, 200 μL	0:50
	DNA Cells LV ¹											Up to 1x10⁶ cultured cells in 200 μL PBS			100, 200 μL	1:20
	DNA Tissue LV ²												Up to 25 mg in 200 μL		200 μL	1:20
Cell-free NA	cfNA ss 2000 ² Protocol optimized for single-stranded DNA			2000 μL											50, 100 μL	2:00
	cfNA ss 4000 ² Protocol optimized for single-stranded DNA			4000 μL											50, 100 μL	2:20
	cfNA ds 2000 ² Protocol optimized for single-stranded DNA			2000 μL											100, 200 μL	2:20
	cfNA ds 4000 ² Protocol optimized for single-stranded DNA			4000 μL											100, 200 μL	2:50
Total RNA	Cellular RNA LV ³											Up to 1x10⁶ cultured cells in 200 μL PBS			50, 100, 200 μL	1:30
	RNA Blood LV 400 ³	900 μL stabilized whole blood from 400 μL whole blood													50, 100, 200 μL	1:30
	RNA Blood LV 800 ³	1800 μL stabilized whole blood from 800 μL whole blood													100, 200 μL	1:30
	RNA PAXgene LV ³	PAXgene pellet in 400 μL PBS													100, 200 μL	1:30
	RNA PAXgene Half Tube LV ³	Half PAXgene pellet in 200 μL PBS													50, 100 μL	1:30
	RNA Tissue FF Standard LV ³												Up to 10 mg in 350 μL		50, 100, 200 μL	1:30
	RNA Tissue FF High LV ³												Up to 25 mg in 700 μL		100, 200 μL	1:30
	RNA Tissue FFPE LV ³													150 μL lysate from 2x5 μm sections	50, 100 μL	1:30
Liquid handling	Sample Transfer to the processing cartridge	50 µL to 1000 µL														NA
	Archive Plate Transfer for archiving purposes	5 μL to 200 μL														NA
	PCR Setup with Mixing															NA
	PCR Setup without Mixing															NA
¹MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001) ² MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001) ³MagNA Pure 96 Cellular RNA Large Volume Kit (cat no. 05 467 535 001) ^{To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set (Cat. no. 07 794 398 001), MagNA Pure External Lysis Buffer (Cat. no. 06 374 913 001), MagNA Pure Bacterial Lysis Buffer (Cat. no. 06 374 921 001), MagNA Pure DNA Tissue Lysis Buffer (Cat. no. 06 640 702 001), MagNA Pure FFPET Buffer Set† (Cat. no. 08 447 144 001) and S.T.A.R. Buffer§ (Cat. no. 03 335 208 001). Please refer to the instructions for use manual for more details.}						^BAL - Bronchoalveolar Lavage ^FFPET - formalin-fixed paraffin-embedded tissue ^**These times are approximate and should only be used as a guidance. ^♰For general laboratory use. ^§For life science research only. Not for use in diagnostic procedures.

MagNA Pure 96 System

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Features

Benefits

Specifications

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