LightCycler^® 96 Instrument

For life science research only. Not for use in diagnostics procedures.

Innovative. Intuitive. Accurate. Dependable.

For today's academic research labs, the new Roche LightCycler^®96 System is the optimal mid-size, mid-throughput real-time PCR solution that conveniently delivers the highest standards expected from a LightCycler^® System: An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user.

The LightCycler^®96 Instrument is a real-time PCR instrument for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping.

Precise LightCycler^® 96 Instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time.

The LightCycler^® 96 Instrument’s optical detection system flexibly detects sequence-dependent probes (i.e., hydrolysis probes) and sequence-independent dyes (i.e., SYBR Green I). Multiplex and multicolor capabilities permit the use of up to four different fluorescent dyes*.

The innovative LightCycler^®96 Application and Instrument Software creates a simple analysis workflow with easy import and export functions, email notifications after each run, online monitoring flexibility, and server-based network interfacing

Features

Plate-based real-time PCR instrument with 96-well block
Temperature gradient (assay optimization)
Standalone system (integrated PC)
Touchscreen with separate instrument software
Dynamic range of 10 log
Sensitivity down to one copy
4-plex multicolor
Runtime of less than 1 hour
Applications: Absolute Quantification, Relative Quantification, Endpoint Genotyping, Melting Curve Analysis, High Resolution Melting
Intuitive state-of-the-art license-free software

Applications

The LightCycler^®96 Instrument is a fast, precise 96-well real-time PCR device. It provides various methods for gene expression and genetic variation analysis in mono- or multicolor (up to 4 colors*) mode.

Primary applications include gene expression analysis, gene detection, mutation detection, methylation analysis, miRNA research, and relative quantification of target genes.

The software includes analysis modules for:

Relative Quantification
Absolute Quantification
Melting Peak
Endpoint Genotyping
High Resolution Melting
Qualitative Detection

Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and SNP analysis with the versatile, plate-based LightCycler^® 96 Instrument:

Benefit from a cutting-edge, low-mass thermal block design that offers excellent well-to-well homogeneity for a broad range of temperatures, as well as access to gradient features. The electroforming production process allows a perfect fit of the 96-well plate (negative structure) to the block (positive structure, formed by a galvanic reaction) and avoids air cushions in between both
Maximize sensitivity and speed using novel optics (sturdy glass fibers combined with high-intensity broad-spectrum LEDs), and obtain simultaneous detection of all wells within milliseconds.
Install the software on a separate computer with no license requirements, and run the system according to your specific needs:
- Standalone, using a flash drive to transfer data
- Connected to a PC
- Connected to a PC network via Ethernet
Experience advanced software features, such as on-line real-time remote monitoring, e-mail notification, heat-maps, wizards, and many more options to operate the intuitive and easy-to-use software.
Extract meaningful data via state-of-the-art analysis methods, then easily export results in a ready-to-publish format (RDML).
Choose the optimal type of LightCycler^® 480 Multiwell Plate or 8-Tube strips to best fit your assay format, application, and workflow. Both white (recommended) and clear formats are available.

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General

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Weight	approx. 55 lb (25 kg)
Dimensions	W x D x H: 16 in x 16 in x 21 in (40 cm x 40 cm x 53 cm)
Noise level during run	43 dB(A)

Hardware

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Thermal cycling system	Peltier-based, 96-well block
Max ramp rate heating	4.4°C/s
Average ramp rate cooling	2.2°C/s
Programmable temperature range	37-98°C
Temperature accuracy	±0.2°C of programmed target temperature
Tm uniformity	Range (max-min) 0.4°C; STD <0.1
Gradient function	Yes
Gradient operational range	37-98°C
Gradient programmable span (X-Y °C)	Max. 20°C gradient range
Excitation	High-power broad spectrum LED
Measurement (Integration time)	Simultaneous data acquisition for all positions in 10-1000 ms (dynamic mode)
Detection	CCD Camera
Optical system	Fixed fiber optics with four excitation and four emission filters No scanning technologies
Cq uniformity	Range (max-min) 0.8; STD <0.2 (enabling resolution of 2-fold concentration differences)

Run Mode

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Standalone	Touchscreen support for instrument configuration Flexible experiment programming and execution Online fluorescence display
PC connected	PC monitoring, analysis tools, and programming
LAN connected	Support of online monitoring using LAN connection Support of remote Roche Service
External devices	Support of external barcode scanner using USB connection
Instrument active communication	Email notification, with success or failure messaging and optional experiment file attachment
Electrical approvals	CE, ICE, UL
Reaction volumes	10-50 μl
Sample format	96-well, 8-tube strips
Runtime	<40 min for 3-step, 40-cycle PCR
Melting measurement	Continuous with 1-25 data points/°C

Applications

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Licensed for real-time PCR	Yes
Dynamic range	10 orders of magnitude
Range of excitation/emission wavelengths (X-Y nm)	470/514 (SYBR, FAM, ResoLight dye) 533/572 (VIC, Hex, Yellow555) 577/620 (Red610, Texas Red) 645/697 (Cy5)
Detection formats	Intercalating dyes (SYBR Green I, ResoLight dye): Hydrolysis Probes
Multiplex analysis (e.g., up to X targets per well)	Up to 4 channels, pre-calibrated color compensation (no user interaction necessary) All channels can be viewed by the software in one chart simultaneously or viewed in 4 charts separately on one screen
Additional dye available	For mono color experiments, any dye between 500 and 720 nm emission wavelength can be used For dual color experiments, only pre-calibrated dyes are usable
Passive reference dyes (e.g., Rox) necessary	No

Analysis Software

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Operating systems	Win 7 or Win XP
Data analysis	Absolute Quantification Relative Quantification Tm Calling Endpoint Genotyping Qual Detection * HRM * (* Coming Soon)
Data export	Result table: export as .txt file Charts: Data and image export
Data import	Sample information can be imported from Excel, .csv, or .txt files
Failure flagging	Automated flagging of critical controls (e.g., positive control is negative or negative control is positive) User definable flagging for further criteria (e.g., standard curve with error greater than X)

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Product	Cat. No.	Content
LightCycler® 96 Instrument	05 815 916 001	1 Instrument
LightCycler® 480 Multiwell Plate 96 (white)	04 729 692 001	50 plates / 50 foils
LightCycler® 96 8-Tube Strips (white)	06 612 601 001	120 strips (white) and optically flat caps (clear); 10 unit packs with 12 tube/cap strips each

To ask a question

LightCycler­® 96 Instrument

Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and SNP analysis with the versatile, plate-based LightCycler® 96 Instrument:

General

General

Hardware

Hardware

Run Mode

Run Mode

Applications

Applications

Analysis Software

Analysis Software

LightCycler^® 96 Instrument

Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and SNP analysis with the versatile, plate-based LightCycler^® 96 Instrument: