LightCycler­® 96 Instrument

For life science research only. Not for use in diagnostics procedures.

AVENIO Edge System
Innovative. Intuitive. Accurate. Dependable.

For today's academic research labs, the new Roche LightCycler® 96 System is the optimal mid-size, mid-throughput real-time PCR solution that conveniently delivers the highest standards expected from a LightCycler® System: An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user.

The LightCycler® 96 Instrument is a real-time PCR instrument for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping.

Precise LightCycler® 96 Instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time.

The LightCycler® 96 Instrument’s optical detection system flexibly detects sequence-dependent probes (i.e., hydrolysis probes) and sequence-independent dyes (i.e., SYBR Green I). Multiplex and multicolor capabilities permit the use of up to four different fluorescent dyes*.

The innovative LightCycler® 96 Application and Instrument Software creates a simple analysis workflow with easy import and export functions, email notifications after each run, online monitoring flexibility, and server-based network interfacing

Lightcycler 96
  • Plate-based real-time PCR instrument with 96-well block
  • Temperature gradient (assay optimization)
  • Standalone system (integrated PC)
  • Touchscreen with separate instrument software 
  • Dynamic range of 10 log
  • Sensitivity down to one copy
  • 4-plex multicolor
  • Runtime of less than 1 hour
  • Applications: Absolute Quantification, Relative Quantification, Endpoint Genotyping, Melting Curve Analysis, High Resolution Melting
  • Intuitive state-of-the-art license-free software

The LightCycler® 96 Instrument is a fast, precise 96-well real-time PCR device. It provides various methods for gene expression and genetic variation analysis in mono- or multicolor (up to 4 colors*) mode.

Primary applications include gene expression analysis, gene detection, mutation detection, methylation analysis, miRNA research, and relative quantification of target genes.

The software includes analysis modules for:

  • Relative Quantification
  • Absolute Quantification
  • Melting Peak
  • Endpoint Genotyping
  • High Resolution Melting
  • Qualitative Detection
Lightcycler 96 features

Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and SNP analysis with the versatile, plate-based LightCycler® 96 Instrument:

  • Benefit from a cutting-edge, low-mass thermal block design that offers excellent well-to-well homogeneity for a broad range of temperatures, as well as access to gradient features. The electroforming production process allows a perfect fit of the 96-well plate (negative structure) to the block (positive structure, formed by a galvanic reaction) and avoids air cushions in between both
  • Maximize sensitivity and speed using novel optics (sturdy glass fibers combined with high-intensity broad-spectrum LEDs), and obtain simultaneous detection of all wells within milliseconds.
  • Install the software on a separate computer with no license requirements, and run the system according to your specific needs:
    • Standalone, using a flash drive to transfer data
    • Connected to a PC
    • Connected to a PC network via Ethernet
  • Experience advanced software features, such as on-line real-time remote monitoring, e-mail notification, heat-maps, wizards, and many more options to operate the intuitive and easy-to-use software.
  • Extract meaningful data via state-of-the-art analysis methods, then easily export results in a ready-to-publish format (RDML).
  • Choose the optimal type of LightCycler® 480 Multiwell Plate or 8-Tube strips to best fit your assay format, application, and workflow. Both white (recommended) and clear formats are available.
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approx. 55 lb (25 kg)


W x D x H: 16 in x 16 in x 21 in (40 cm x 40 cm x 53 cm)

Noise level during run

43 dB(A)


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Thermal cycling system

Peltier-based, 96-well block

Max ramp rate heating


Average ramp rate cooling


Programmable temperature range


Temperature accuracy

±0.2°C of programmed target temperature


Range (max-min) 0.4°C; STD <0.1

Gradient function


Gradient operational range


Gradient programmable span (X-Y °C)

Max. 20°C gradient range


High-power broad spectrum LED

Measurement (Integration time)

Simultaneous data acquisition for all positions in 10-1000 ms (dynamic mode)


CCD Camera

Optical system

Fixed fiber optics with four excitation and four emission filters

No scanning technologies

Cq uniformity

Range (max-min) 0.8; STD <0.2 (enabling resolution of 2-fold concentration differences)

Run Mode

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Run Mode



Touchscreen support for instrument configuration

Flexible experiment programming and execution

Online fluorescence display

PC connected

PC monitoring, analysis tools, and programming

LAN connected

Support of online monitoring using LAN connection

Support of remote Roche Service

External devices

Support of external barcode scanner using USB connection

Instrument active communication

Email notification, with success or failure messaging and optional experiment file attachment

Electrical approvals


Reaction volumes

10-50 μl

Sample format

96-well, 8-tube strips


<40 min for 3-step, 40-cycle PCR

Melting measurement

Continuous with 1-25 data points/°C


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Licensed for real-time PCR


Dynamic range

10 orders of magnitude

Range of excitation/emission wavelengths (X-Y nm)

470/514 (SYBR, FAM, ResoLight dye)

533/572 (VIC, Hex, Yellow555)

577/620 (Red610, Texas Red)

645/697 (Cy5)

Detection formats

Intercalating dyes (SYBR Green I, ResoLight dye): Hydrolysis Probes

Multiplex analysis (e.g., up to X targets per well)

Up to 4 channels, pre-calibrated color compensation (no user interaction necessary) All channels can be viewed by the software in one chart simultaneously or viewed in 4 charts separately on one screen

Additional dye available

For mono color experiments, any dye between 500 and 720 nm emission wavelength can be used

For dual color experiments, only pre-calibrated dyes are usable

Passive reference dyes (e.g., Rox) necessary


Analysis Software

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Analysis Software


Operating systems

Win 7 or Win XP

Data analysis

Absolute Quantification

Relative Quantification


Endpoint Genotyping

Qual Detection *


(* Coming Soon)

Data export

Result table: export as .txt file Charts: Data and image export

Data import

Sample information can be imported from Excel, .csv, or .txt files

Failure flagging

Automated flagging of critical controls (e.g., positive control is negative or negative control is positive)

User definable flagging for further criteria (e.g., standard curve with error greater than X)

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Product Cat. No.

LightCycler® 96 Instrument

05 815 916 001

1 Instrument

LightCycler® 480 Multiwell Plate 96 (white)

04 729 692 001

50 plates / 50 foils 

LightCycler® 96 8-Tube Strips (white)

06 612 601 001

120 strips (white) and optically flat caps (clear); 10 unit packs with 12 tube/cap strips each

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