The KAPA NGS FFPE DNA QC Kit offers a reliable qPCR-based qualification and recommended input assessment of human FFPE DNA samples prior to NGS library construction. Each kit contains an optimized high performing qPCR Master Mix, a DNA QC standard and primers in premixes formats. The primers target the human Long Interspersed Nuclear Elements (LINE) offering increased sensitivity and minimizing the amount of sample needed for QC.
Sample quality is assessed by relative quantification across a short and a longer amplicon, compared to the DNA QC standard. The calculated normalized Quality score (Q-score) is used to calculate the required input amount of the sample, which offers the greatest probability for high quality library preparation. Q-scores generated with the kit may be used to predict the outcome of library preparation and optimize library construction parameters for FFPE DNA samples.
The KAPA FFPE DNA QC Kit is validated in the KAPA HyperPETE Tissue DNA Workflow but may be theoretically compatible with the KAPA HyperCap FFPET DNA Workflow.