For Research Use Only. Not for use in diagnostic procedures.
Monitor the sequence of the SARS-CoV-2 virus and the emergence of new variants with KAPA SARS-CoV-2 Target Enrichment Panel. Sequence viral genomes from fresh or degraded (e.g., wastewater) samples with a panel targeting 100% of the reference SARS-CoV-2 genome (NC_045512) and >99.7% of another 183 publicly available SARS-CoV-2 genomes (GenBank).
Figure 2. KAPA SARS-CoV-2 panel achieves more uniform coverage compared to the ARTIC amplicon method. IGV coverage plot of one representative replicate of the KAPA SARS-CoV-2 TE dataset (green) compared to a replicate from the ARTIC workflow with v3 primers (gray). A near dropout of reads (black box) was observed in the Spike gene for the ARTIC libraries in all 3 replicates tested while the KAPA SARS-CoV-2 TE yielded more even coverage across the whole Spike region.
Shortening the hybridization time to 1 hour yields equivalent 1X SARS-CoV-2 genome coverage compared to 16-hour hybridization. Libraries generated from samples containing the indicated number of viral copies in a background of 20 ng of human RNA were hybridized to the SARS-CoV-2 target enrichment panel for 1 hour or 16 hours and processed using the HyperCap Workflow v3 in triplicate. A. The 1-hour hybridization time and 16-hour hybridization time yielded similar SARS-CoV-2 genome coverage at 1X for all viral copy levels. B. Samples with higher viral loads also yielded similar genome coverage at 20X for the 1-hour and 16-hour hybridization times, with somewhat reduced coverage for lower viral loads.
Figure 3. Libraries generated from samples containing the indicated number of viral copies in a background of 20 ng of human RNA were hybridized to the SARS-CoV-2 target enrichment panel for 1 hour or 16 hours and processed using the HyperCap Workflow v3 in triplicate. A. The 1-hour hybridization time and 16-hour hybridization time yielded similar SARS-CoV-2 genome coverage at 1X for all viral copy levels. B. Samples with higher viral loads also yielded similar genome coverage at 20X for the 1-hour and 16-hour hybridization times, with somewhat reduced coverage for lower viral loads.
Fig 4. Libraries generated from samples containing the indicated number of viral copies in a background of 20 ng of human RNA were hybridized to the SARS-CoV-2 target enrichment panel for 1 hour or 16 hours and processed using the HyperCap Workflow v3 in triplicate. A. The 1-hour hybridization time and 16-hour hybridization time yielded similar SARS-CoV-2 genome coverage at 1X for all viral copy levels. B. Samples with higher viral loads also yielded similar genome coverage at 20X for the 1-hour and 16-hour hybridization times, with somewhat reduced coverage for lower viral loads.
The KAPA SARS-CoV-2 Target Enrichment Panel achieves 1X coverage of >97% of the SARS-CoV-2 genome down to 1000 viral copies and genomic sequence from as few as 10 viral copies. Samples containing the indicated number of viral copies in a background of either 20 ng or 100 ng of human RNA background were processed in triplicate using KAPA RNA HyperPrep and the KAPA SARS-CoV-2 Target Enrichment Panel. Datasets were downsampled to 1 million reads prior to analysis.
Figure 5. The KAPA SARS-CoV-2 Target Enrichment Panel achieves 1X coverage of >97% of the SARS-CoV-2 genome down to 1000 viral copies and genomic sequence from as few as 10 viral copies. Samples containing the indicated number of viral copies in a background of either 20 ng or 100 ng of human RNA background were processed in triplicate using KAPA RNA HyperPrep and the KAPA SARS-CoV-2 Target Enrichment Panel. Datasets were downsampled to 1 million reads prior to analysis.
Figure 6. The KAPA SARS-CoV-2 Target Enrichment Panel identifies the mutations associated with 6 variants in a single mixed sample. A total of 1,000,000 SARS-CoV-2 genome copies from 6 different variants was combined with either 20 ng (dark blue) or 100 ng (yellow) of human RNA and processed in triplicate using the KAPA RNA HyperPrep Kit and the KAPA SARS-CoV-2 Target Enrichment Panel with the standard 16-hour hybridization. Alternate allele frequency analysis shows that all expected mutations from the six SARS-CoV-2 variants were identified at close to the expected frequency. Datasets downsampled to 1 million reads prior to analysis.
| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| 09436499001 |
09436499001 |
KAPA HyperCap SARS-CoV-2 panel 12 rxn |
12 rxn |
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| 09436502001 |
09436502001 |
KAPA HyperCap SARS-CoV-2 panel 24 rxn |
24 rxn |
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| 09436529001 |
09436529001 |
KAPA HyperCap SARS-CoV-2 panel 48 rxn |
48 rxn |
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| 09436537001 |
09436537001 |
KAPA HyperCap SARS-CoV-2 panel 96 rxn |
96 rxn |
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Please contact your local Roche representative for detailed ordering information.
