KAPA Taq PCR Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA Taq PCR Kits

Overview
 

The Kapa Taq DNA polymerase enzyme is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. Kapa Taq and Kapa Taq HotStart DNA polymerase enzymes have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated.

 

Features and Benefits of KAPA Taq PCR Kits
 

  • Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step
  • Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
  • Supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template
  • Also available with loading dye reaction buffer, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye

Product highlights

Improved sensitivity, specificity and yield compared to other available kits
  • Novel buffer formulation, facilitating specific primer annealing, leading to higher yield of specific product

 

View data showing superior PCR performance with the amplification of CCR5 gene fragment using KAPA Taq PCR Kit compared to competitor kits.

See data showing higher sensitivity and less primer dimers using Kapa HotStart enzyme compared to KAPA Taq PCR Kit from mycoplasma DNA fragment amplification.

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Specifications

  • Starting Material

    DNA, Plasmid DNA, cDNA

  • Input Amount

    ≤250 ng for genomic DNA; ≤25 ng for less complex DNA

Kits can be stored for up to 18 months at -20˚C.

Kits include KAPA Taq (or KAPA Taq HotStart) DNA Polymerase (5 U/µL), KAPA Taq Buffer, and MgCl2 (25 mM). Kits are available with and without dNTP Mix (10 mM each). The following reaction buffers are available:

  • KAPA Taq Buffer A and B (10X, with 1.5 mM MgCl2 at 1X), supplied with KAPA Taq PCR Kits only
  • KAPA Taq Buffer with dye (10X, with 1.5 mM MgCl2 at 1X), supplied with KAPA Taq PCR Kits with dye only
  • KAPA Taq HotStart Buffer (5X, Mg-free), supplied with KAPA Taq HotStart PCR Kits only

KAPA Taq HotStart DNA Polymerase is also sold separately.

ReadyMix formulations (2X) of KAPA Taq DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with and without dye), dNTPs and MgCl2 (at a final concentration of 1.5 mM).

Contents

Components
KAPA Taq Standard or HotStart DNA Polymerase 5 U/µL
10X KAPA Taq Buffer A
10X KAPA Taq Buffer B
10X KAPA Buffer with loading dye (optional)
5X KAPA Taq HotStart Buffer (HotStart kits only)
MgCl(25 mM)
dNTP Mix (10 mM each; optional)
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Component

Kit Code Roche Cat. No
Description
Kit Size
BK1000
07958447001
KAPA Taq PCR Kits - Standard kit
2500 U
BK1001
07958455001
KAPA Taq PCR Kits - With dNTPs
2500 U
BK1002
07958463001
KAPA Taq PCR Kits - Standard kit
5000 U
BK1003
07958471001
KAPA Taq PCR Kits - With dNTPs
5000 U
BK1004
07958498001
KAPA Taq PCR Kits - With dye
2500 U
BK1005
07958501001
KAPA Taq PCR Kits - With dye, with dNTPs
2500 U
BK1006
07958510001
KAPA Taq PCR Kits - With dye
5000 U
BK1007
07958528001
KAPA Taq PCR Kits - With dye, with dNTPs
5000 U
KK1008
07958552001
KAPA Taq PCR Kits - With dNTPs
250 U
KK1014
07958579001
KAPA Taq PCR Kits - Standard kit
250 U
KK1015
07958587001
KAPA Taq PCR Kits - Standard kit
500 U
KK1016
07958595001
KAPA Taq PCR Kits - With dNTPs 500 U
KK1020
07958617001
KAPA Taq PCR Kits - With dye
250 U
KK1021
07958625001
KAPA Taq PCR Kits - With dye, with dNTPs
250 U
KK1022
07958633001
KAPA Taq PCR Kits - With dye
500 U
KK1023
07958641001
KAPA Taq PCR Kits - With dye, with dNTPs
500 U
KK1508
07958765001
KAPA Taq PCR Kits - HotStart PCR Kit
250 U
KK1509
07958773001
KAPA Taq PCR Kits - HotStart PCR Kit, with dNTPs
250 U
KK1510
07958781001
KAPA Taq PCR Kits - HotStart PCR Kit 500 U
KK1511
07958790001
KAPA Taq PCR Kits - HotStart PCR Kit, with dNTPs
500 U
KK1512
07958803001
KAPA Taq PCR Kits - HotStart DNA polymerase, enzyme only
2500 U
KK1513
07958811001
KAPA Taq PCR Kits - HotStart PCR Kit
2500 U

ReadyMix

Kit Code Roche Cat. No
Description
Kit Size
KK1006
07958536001
KAPA Taq PCR Kits - ReadyMix, 250 x 50 µL reactions
6.25 mL
KK1024
07958650001
KAPA Taq PCR Kits - ReadyMix with dye, 250 x 50 µL reactions
6.25 mL
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