The KAPA RNA EvoPrep Kits (with no upfront enrichment) and the KAPA RNA EvoPrep Kits with RiboErase (HMR) and/or RiboErase (HMR) Globin are compatible with RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue. The quality of FFPE-derived RNA can be highly variable due to the damaging nature of the formalin fixation process, where crosslinking, chemical modification, and fragmentation can occur. Library construction results may vary depending on the input amount and quality of the RNA. Higher RNA inputs may salvage library construction for particularly difficult FFPE samples. Please refer to the recommendations outlined in the Instructions for Use for each kit
KAPA mRNA EvoPrep Kits are only suitable for mRNA capture and library construction from high-quality input material (RIN ≥ 7). The use of fragmented RNA will result in strong bias towards the 3’-end of the mRNA.
The KAPA RNA EvoPrep Kits (with no upfront enrichment) and the KAPA RNA EvoPrep Kits with RiboErase (HMR) and/or RiboErase (HMR) Globin are compatible with RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue. The quality of FFPE-derived RNA can be highly variable due to the damaging nature of the formalin fixation process, where crosslinking, chemical modification, and fragmentation can occur. Library construction results may vary depending on the input amount and quality of the RNA. Higher RNA inputs may salvage library construction for particularly difficult FFPE samples. Please refer to the recommendations outlined in the Instructions for Use for each kit
KAPA mRNA EvoPrep Kits are only suitable for mRNA capture and library construction from high-quality input material (RIN ≥ 7). The use of fragmented RNA will result in strong bias towards the 3’-end of the mRNA.
Specs
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KAPA RNA EvoPrep Kit
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KAPA mRNA EvoPrep Kit
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KAPA RNA EvoPrep Kit with RiboErase (HMR)
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KAPA RNA EvoPrep Kit with RiboErase (HMR) Globin (for blood samples)
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Input
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0.25 ng – 100 ng
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25 ng – 1 μg
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5 ng – 1 μg
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5 ng – 1 μg
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Input type
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High-quality total RNA
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Recommended
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Low-quality total RNA (e.g. FFPE RNA)
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Compatible
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Not Recommended
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Compatible
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Compatible
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Poly(A)-selected and/or depleted RNA
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Recommended
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N/A
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N/A
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N/A
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