The KAPA HyperCap Oncology panel is a 214 Kb capture target panel covering the full coding sequence of 13 genes related to somatic oncology research (ATM, BRCA1, BRCA2, BRIP1, CHEK2, EGFR, ERBB2, KRAS, MET, MYCN, RAD51C, RAD51D, TP53) as well as hotspot variants across 69 relevant genes, which are found in commercially available controls. It is readily available from stock and it offers consistent quality by NGS probes QC of every lot. It is optimized to deliver high uniformity and high specificity from FFPET and cfDNA samples.
Figure 1. Key sequencing metrics with the KAPA HyperCap Oncology Panel. Two real-world low-quality FFPET DNA samples were compared with the Horizon™ HD789 control FFPE DNA sample. Sample libraries (in duplicates) were prepared following the KAPA HyperCap FFPET DNA Workflow from 10 ng FFPE DNA with the KAPA HyperPlus Kit. Following single library hybridization and capture, the final libraries were sequenced on a NextSeq™ 550 system and downsampled to 10 M HQ filtered clusters (2 x 150 bp) prior to analysis.
Figure 2. Five healthy donor cfDNA samples were tested in duplicate (average is shown), in parallel with SeraSeq® ctDNA Complete™ Reference Material AF0.5% (duplicates) starting from 10 ng of input. The KAPA HyperCap cfDNA Workflow was followed using the KAPA HyperPrep Kit and single sample library hybridization and captures. Final libraries were sequenced on a NovaSeq™ 6000 system. Fifty (50) million high-quality filtered clusters (2 x 150 bp) were analyzed per sample
Demo data is available for evaluation. Please contact your local Roche representative.
Roche Catalog# | Description | Kit size (Reaction) | How to buy |
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09462384001 | KAPA Oncology panel 12 rxn | 12 | Contact us |
09462457001 | KAPA Oncology panel 24 rxn | 24 | Contact us |
09462465001 | KAPA Oncology panel 48 rxn | 48 | Contact us |