KAPA HyperCap Heredity Panel

KAPA HyperCap Heredity Panel offers high performance

The KAPA HyperCap Heredity Panel is a 10 Mb capture target panel covering 3332 genes strongly associated with hereditary disease research, plus the ClinVar pathogenic and likely pathogenic variants content. It provides an optimal balance between relevant content carefully selected by the Roche Sequencing Scientists and sequencing efficiency driven from exceptional uniformity and very low duplication rates. It is readily available from stock and offers consistent quality by NGS probes QC of every lot.

Elevate sequencing efficiency

Achieve a fine balance of high specificity (80.9% reads on-target), high uniformity (1.42 Fold-80 base penalty) and low PCR duplication (2.2%)
Maximize your sequencing efficiency to cover 98.5% of the target bases by 30x and achieve a mean coverage of 92.6x
Uncover efficiencies across the sample preparation process with the streamlined and automation-friendly KAPA HyperCap Workflow

Figure 1. Key sequencing metrics with the KAPA HyperCap Heredity Panel. Eight-plex pre-capture multiplexing with the standard KAPA HyperCap Workflow v3 including the KAPA HyperPlus Kit from 100 ng gDNA (16 coriell cell lines in duplicate). Final libraries were sequenced on a NovaSeq™ 6000 system and downsampled to 10 M HQ filtered clusters (2 x 100 bp) prior to analysis.

Sequence what matters

Rely on Roche’s renowned content and design expertise to focus on relevant up-to-date content
Cover more than 85% of the total ClinVar Pathogenic and likely pathogenic content at >30x by targeting those carefully selected 3332 genes in a sequencing efficient 10 Mb capture target
Achieve high percentage of bases covered by 30x for the ACMG 73 genes and the ENSEMBL and RefSeq transcripts of the 3332 genes targeted by the KAPA HyperCap Heredity Panel

Figure 2. Percent bases covered at 30x and above in key genomic databases using the KAPA HyperCap Heredity Panel. Twelve-plex precapture multiplexing with the standard KAPA HyperCap Workflow v3 and the KAPA HyperPrep Kit from 100 ng gDNA (96 replicates, NA12878). Final libraries were sequenced on a NovaSeq™ 6000 system and downsampled to 10 M high-quality filtered clusters (2 x 100 bp) prior to analysis.

Demo data

Demo data is available for evaluation. Please contact your local Roche representative.

To place an order or ask a question

Cat Number	Description	Reaction pack size
09462473001	KAPA Heredity panel 12 rxn	12
09462481001	KAPA Heredity panel 24 rxn	24
09462490001	KAPA Heredity panel 48 rxn	48
09462503001	KAPA Heredity panel 96 rxn	96
09462511001	KAPA Heredity panel 192 rxn	192

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KAPA HyperCap Heredity Panel