Overview

As the first step in the NGS workflow continuum, sample prep holds the key to unlocking the potential of every sample. Roche Sample Prep Solutions provide an adaptable, reliable and focused portfolio of high-performance, high-quality library preparation reagents that enable you to process more samples successfully, obtain more information from every sample, and optimize your sequencing resources. This includes QC-tested adapters of different designs to meet the needs of a wide range of multiplexed DNA and RNA sequencing applications.

Benefits of KAPA Adapters

  • Different adapter designs and indexing strategies to support a wide range of multiplexed sequencing applications
  • Low (≥2-plex) to high (up to 384-plex) levels of sequencing multiplexing are supported on one-, two-, and four-channel Illumina® instruments, on patterned and non-patterned flow cells
  • Easy to incorporate in new and existing Illumina sequencing workflows
  • Functionally tested to confirm high library construction efficiency and minimal levels of adapter-dimer formation
  • Sequencing-based QC testing reduces the potential contribution of barcode cross-contamination to index misassignment
  • Compatible with KAPA DNA and KAPA RNA library preparation kits, for manual or automated library construction

KAPA Adapters Features

The current portfolio of KAPA Adapters comprises 96 high-quality, ready-to-use, full-length unique dual-indexed (UDI) adapters and 384 high-quality truncated unique dual-indexed adapters with or without unique molecular identifiers (UMI), for ligation-based library construction in Illumina sequencing workflows:

KAPA Unique Dual-Indexed (UDI) Adapters contain non-redundant sequencing barcode combinations, designed to mitigate the impact of index misassignment on Illumina instruments with patterned flow cells.

KAPA HyperPlex Adapters offering includes a truncated adapter with or without UMI, supported with 384 UDI primer mixes that contain non-redundant sequencing barcode combinations, designed to mitigate the impact of index misassignment on Illumina instruments with patterned flow cells.

KAPA Adapter Selection Guide table

1The sets of 192 and 768 barcode sequences used in KAPA UDI Adapters and KAPA UDI Primer Mixes (respectively) are exclusive to Roche and different between the two sets.

2Theoretically possible but not fully tested.

3This is sufficient for four or one library preps (KAPA UDI Adapter plate or KAPA UDI Primer Mixes plate, respectively) with the KAPA HyperPrep or KAPA HyperPlus Kit if no adapter dilution is required. Generous overfill supports use on automated liquid handling systems.

4KAPA UDI Primer Mixes and KAPA UDI Adapter plates are shipped with peelable seals. Replacement seals (three per plate) are peelable and pierceable.

*KAPA HyperPlex Adapters and KAPA UDI Adapters are not compatible with methyl-seq applications.

 

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KAPA UDI Adapters

High-quality full length UDI adapters for multiplexed sequencing on 1- , 2- and 4-channel Illumina instruments, with patterned or non-patterned flow cells. Compatible with all KAPA Hyper library preparation kits.

Learn more
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KAPA HyperPlex Adapters

High-quality truncated adapters with or without UMI and 384 UDI Primer mixes for multiplexed sequencing on 1- , 2- and 4-channel Illumina instruments, with patterned or non-patterned flow cells. Compatible with all KAPA Hyper library preparation kits.

Learn more