Specifications

Roche Sequencing Solutions offers a family of KAPA HiFi Kits, containing the same core, engineered KAPA HiFi DNA Polymerase enzyme, formulated for both NGS and PCR applications. These kits are available in different formats and formulations, to provide flexibility and convenience across a broad range of applications.

The table below describes the specifications for the KAPA HiFi Kits:

	KAPA HiFi PCR Kit	KAPA HiFi HotStart PCR Kit	KAPA HiFI HotStart ReadyMix PCR Kit	KAPA HiFi HotStart Library Amplification Kit	KAPA HiFi HotStart Library Amplification Kit with Primer Mix	KAPA HiFi HotStart Real-Time Library Amplification Kit
HotStart Enzyme	No	Yes	Yes	Yes	Yes	Yes
Format	Components	Components	ReadyMix	ReadyMix	ReadyMix with Primer Mix	ReadyMix
Kit Contents	KAPA HiFi enzyme KAPA HiFi buffers Magnesium Chloride dNTPs	KAPA HiFi HotStart enzyme KAPA HiFi buffers Magnesium Chloride dNTPs	KAPA HiFi HotStart ReadyMix	KAPA HiFi HotStart ReadyMix	KAPA HiFi HotStart ReadyMix Amplification Primer Mix	KAPA HiFi HotStart ReadyMix Fluorescein Standards
Recommended Applications	PCR applications requiring flexibility and/or optimization	PCR applications requiring a HotStart enzyme and flexibility and/or optimization	Routine use in established PCR and NGS assays	Routine NGS library amplification on any platform using user-supplied primers	Routine NGS library amplification for Illumina® platforms	NGS library amplification for Illumina® platforms that require careful control

Research Use Only. Not for use in diagnostic procedures.
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