Roche Sequencing Solutions offers a family of KAPA HiFi Kits, containing the same core, engineered KAPA HiFi DNA Polymerase enzyme, formulated for both NGS and PCR applications. These kits are available in different formats and formulations, to provide flexibility and convenience across a broad range of applications.

The table below describes the specifications for the KAPA HiFi Kits:

  KAPA HiFi PCR Kit   KAPA HiFi HotStart PCR Kit KAPA HiFI HotStart ReadyMix PCR Kit KAPA HiFi HotStart Library Amplification Kit KAPA HiFi HotStart Library Amplification Kit with Primer Mix KAPA HiFi HotStart Real-Time Library Amplification Kit
HotStart Enzyme No Yes Yes Yes Yes Yes
Format Components Components ReadyMix ReadyMix ReadyMix with Primer Mix ReadyMix
Kit Contents KAPA HiFi enzyme
KAPA HiFi buffers
Magnesium Chloride
dNTPs
KAPA HiFi HotStart enzyme
KAPA HiFi buffers
Magnesium Chloride
dNTPs
KAPA HiFi HotStart ReadyMix KAPA HiFi HotStart ReadyMix
KAPA HiFi HotStart ReadyMix
Amplification Primer Mix
KAPA HiFi HotStart ReadyMix
Fluorescein Standards
Recommended
Applications
PCR applications requiring flexibility and/or optimization PCR applications requiring a HotStart enzyme and flexibility and/or optimization Routine use in established PCR and NGS assays Routine NGS library amplification on any platform using user-supplied primers Routine NGS library amplification for Illumina® platforms NGS library amplification for Illumina® platforms that require careful control