Overview

As next-generation sequencing (NGS) sample preparation entails a multitude of steps and each step is critical for the overall sequencing success and efficiency, all steps need to be optimized in terms of reagents and protocol. Considering the precious nature of NGS samples, an integrated workflow with optimized reagents is necessary converting the samples into a sequencing-ready library. NGS sample types can differ in terms of their:

  • Ease of sequencing - uniform or with secondary structures or GC-rich areas
  • Quality - freshly extracted or preserved (formalin fixed paraffin embedded - FFPE)
  • Flexible Workflows - DNA and RNA workflows optimized for applications

Taking into account all these variations, it is important to use library preparation reagents that can work efficiently with all these sample types. High-quality library preparation reagents help in obtaining uniform and improved coverage as well as lower duplication rates. Depending on the end goal (for example, whole exome sequencing), a target enrichment step may also be required for enriching specific regions of interest. Use of high-efficiency target enrichment reagents helps in obtaining uniform and high depth of coverage with high specificity (on-target rate) over the targeted regions.

The KAPA HyperPETE Workflow combines validated products for both KAPA Library Preparation and KAPA Target Enrichment. It provides all accessories and reagents needed for sample preparation, from DNA or RNA to the sequencer in a single-day workflow. The new KAPA HyperPETE Panels provide  high capture efficiency, sequencing uniformity with less optimization and higher variant detection confidence. In addition, the workflow will include KAPA Universal (with or without UMI - Unique Molecular Indexes) Adapters and KAPA UDI primer mixes (up to 384*). The KAPA HyperPETE Workflow is especially suited for small panel oncology research applications and is supported by an integrated cloud-based analysis solution (NAVIFY® Mutation Caller). NAVIFY Mutation Caller supports KAPA HyperPETE Workflow with pipelines for somatic SNV, Indel and CNV calling, microsatellite instability (MSI) scoring, RNA fusion detection (known and unknown fusion partners) and germline SNV and Indel calling. The KAPA HyperPETE Workflow also provides the convenience of simplified ordering and support from a single vendor, and is automation friendly. 

* KAPA HyperPETE Workflow is validated with the KAPA UDI Primer Mixes 1 – 96 and is theoretically compatible with KAPA UDI Primer Mixes 97 – 384

Features and Benefits of the HyperPETE Workflow

  • Roche's renowned design expertise allows variant detection in difficult genomic regions

  • Validated with challenging sample types to detect all major mutation classes (SNV, CNV, Indels, Fusions including unknown fusion partners and MSI)

  • Optimized to deliver accurate molecule counting and low error rates by using the KAPA Universal UMI Adapter

  • Outstanding hybrid capture performance in small panels by extensive workflow optimization

  • Single-day workflow with short incubations and low hands-on-time

  • Convenient all-inclusive workflow with validated products and simplified ordering and support from a single vendor

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Customization flexibility

  • Leverage content & design expertise from our KAPA Target Enrichment portfolio to uncover difficult regions
  • Fast custom panel ordering turnaround time for rapid deployment and testing

 

All kits & reagents provided for Library Prep and Target Enrichment

  • Single vendor service and support experience
  • Integrated & optimized workflow
  • Economies of scale

** Not available for sale in the United States. Check the product availability with your local Roche representative.

Highly optimized workflow with high performing catalog and custom panels

  • Optimized for somatic variant detection in cfDNA, FFPE DNA and RNA samples
  • Catalog panels
    • Somatic oncology: Hotspot, Pan cancer Lung Cancer Fusion panels
    • Germline: Newborn screening** and Hereditary Onco panels
  • Save precious samples with no panel splitting
  • Superior sequencing efficiency by high uniformity, focused panel design, and no primer trimming

Analysis support for mutation calling

  • NAVIFY Mutation Caller analysis tool assists in customized analysis for KAPA HyperPETE workflows.