As next-generation sequencing (NGS) sample preparation entails a multitude of steps and each step is critical for the overall sequencing success and efficiency, all steps need to be optimized in terms of reagents and protocol. Considering the precious nature of NGS samples, an integrated workflow with optimized reagents is necessary converting the samples into a sequencing-ready library. NGS sample types can differ in terms of their:
Taking into account all these variations, it is important to use library preparation reagents that can work efficiently with all these sample types. High-quality library preparation reagents help in obtaining uniform and improved coverage as well as lower duplication rates. Depending on the end goal (for example, whole exome sequencing), a target enrichment step may also be required for enriching specific regions of interest. Use of high-efficiency target enrichment reagents helps in obtaining uniform and high depth of coverage with high specificity (on-target rate) over the targeted regions.
The KAPA HyperPETE Workflow combines validated products for both KAPA Library Preparation and KAPA Target Enrichment. It provides all accessories and reagents needed for sample preparation, from DNA or RNA to the sequencer in a single-day workflow. The new KAPA HyperPETE Panels provide high capture efficiency, sequencing uniformity with less optimization and higher variant detection confidence. In addition, the workflow will include KAPA Universal (with or without UMI - Unique Molecular Indexes) Adapters and KAPA UDI primer mixes (up to 384*). The KAPA HyperPETE Workflow is especially suited for small panel oncology research applications and is supported by an integrated cloud-based analysis solution (NAVIFY® Mutation Caller). NAVIFY Mutation Caller supports KAPA HyperPETE Workflow with pipelines for somatic SNV, Indel and CNV calling, microsatellite instability (MSI) scoring, RNA fusion detection (known and unknown fusion partners) and germline SNV and Indel calling. The KAPA HyperPETE Workflow also provides the convenience of simplified ordering and support from a single vendor, and is automation friendly.
* KAPA HyperPETE Workflow is validated with the KAPA UDI Primer Mixes 1 – 96 and is theoretically compatible with KAPA UDI Primer Mixes 97 – 384
Roche's renowned design expertise allows variant detection in difficult genomic regions
Validated with challenging sample types to detect all major mutation classes (SNV, CNV, Indels, Fusions including unknown fusion partners and MSI)
Optimized to deliver accurate molecule counting and low error rates by using the KAPA Universal UMI Adapter
Outstanding hybrid capture performance in small panels by extensive workflow optimization
Single-day workflow with short incubations and low hands-on-time
Convenient all-inclusive workflow with validated products and simplified ordering and support from a single vendor