qPCR

Overview

Quantitative real-time PCR (qPCR) enables the detection and quantification of PCR products in real time and is used widely in gene expression studies due to its high sensitivity, reproducibility and wide dynamic range. The technique uses double-stranded DNA (dsDNA)-binding dyes or target-specific fluorescently labeled primers and probes that hybridize with the target sequence to measure the relative amount of DNA. Most commercially available qPCR reagents contain wild-type, unmodified DNA polymerase, but these enzymes were never intended as tools for use in molecular biology. The performance differences among commercial qPCR kits are the result of differences in factors like buffer formulation and/or enzyme concentration.

Roche offers KAPA qPCR reagents containing DNA polymerase and other novel enzymes specifically selected through our directed evolution technology for qPCR applications. These confer significant improvements to reaction efficiency, sensitivity, speed, and signal-to-noise ratio.

Gene Expression

KAPA SYBR FAST qPCR Kits

KAPA SYBR FAST One-Step qRT-PCR Kits

KAPA PROBE FAST qPCR Kits

KAPA PROBE FORCE qPCR Kits

  • Higher sensitivity and improved reaction efficiency across a broad range of amplicon sizes and GC contents*
  • Reduced experimental variation and contamination with a convenient one-step qRT-PCR protocol*
  • Optimized for versatility and speed, providing highly reproducible results for gene expression assays
  • Direct qPCR from crude blood, tissue, and plant extracts.
  • Sample-to-Cq (workflows in <1 hour*)
  • High efficiency for accurate, reproducible, and sensitive results
Genotyping

KAPA SYBR FAST qPCR Kits

KAPA PROBE FAST qPCR Kits

KAPA HRM FAST qPCR Kits

KAPA PROBE FORCE qPCR Kits

  • Higher sensitivity and improved reaction efficiency across a broad range of amplicon sizes and GC contents*
  • Optimized for versatility and speed, compatible with all probe-based SNP detection applications
  • Engineered to maximize differences in melting behavior between sequence variants resulting in accurate SNP genotyping with maximum sensitivity and speed
  • Direct qPCR from crude blood, tissue, and plant extracts.
  • Sample-to-Cq (workflows in <1 hour)
  • High efficiency for accurate, reproducible, and sensitive results
Food and Water Safety
KAPA PROBE FORCE qPCR Kits
  • Direct qPCR from crude blood, tissue, and plant extracts
  • Sample-to-Cq (workflows in <1 hour)
  • High efficiency for accurate, reproducible, and sensitive results

Roche | Sequencing Solutions KAPA SYBR FAST qPCR Kits

Utilize this qPCR kit containing a DNA polymerase specifically engineered for high tolerance to SYBR Green I dye inhibition.

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Roche Sequencing | KAPA SYBR FAST One-Step qRT-PCR Kits

Explore this one-step qPCR kit designed for optimal performance in stringent qRT-PCR reaction conditions.

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Roche | Sequencing Solutions KAPA PROBE FORCE qPCR Kits

Explore this kit containing a highly inhibitor-resistant qPCR master mix that eliminates the need for DNA purification, offering a streamlined sample-to-Cq workflow

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Roche | Sequencing Solutions KAPA PROBE FAST qPCR Kits

Use this kit for all probe-based PCR applications to get accurate and reliable results.

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Roche | Sequencing Solutions KAPA HRM FAST

Use this kit with a novel DNA polymerase and buffer system and the Eva Green dye optimized for maximum discrimination between sequence variations for HRM analysis.

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*Data on file.