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qPCR
Overview
Quantitative real-time PCR (qPCR) enables the detection and quantification of PCR products in real time and is used widely in gene expression studies due to its high sensitivity, reproducibility and wide dynamic range. The technique uses double-stranded DNA (dsDNA)-binding dyes or target-specific fluorescently labeled primers and probes that hybridize with the target sequence to measure the relative amount of DNA. Most commercially available qPCR reagents contain wild-type, unmodified DNA polymerase, but these enzymes were never intended as tools for use in molecular biology. The performance differences among commercial qPCR kits are the result of differences in factors like buffer formulation and/or enzyme concentration.
Roche offers KAPA qPCR reagents containing DNA polymerase and other novel enzymes specifically selected through our directed evolution technology for qPCR applications. These confer significant improvements to reaction efficiency, sensitivity, speed, and signal-to-noise ratio.
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| Genotyping |
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| Food and Water Safety |
KAPA PROBE FORCE qPCR Kits |
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KAPA SYBR FAST qPCR Kits
Containing the first DNA polymerase engineered via directed evolution technology to be more tolerant to SYBR Green I dye inhibition, KAPA SYBR FAST qPCR Kits confer high sensitivity and reaction efficiency across a broad range of amplicon sizes and GC contents.
Learn moreKAPA SYBR FAST One-Step qRT-PCR Kits
Designed for optimal performance in stringent qRT-PCR reaction conditions, KAPA SYBR FAST One-Step qRT-PCR Kits are optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination.
Learn moreKAPA PROBE FORCE qPCR Kits
Containing a highly inhibitor-resistant qPCR master mix with a third-generation DNA polymerase selected through our direct evolution technology, the KAPA PROBE FORCE qPCR Kits remove the need for DNA purification and enable streamlined sample-to-Cq workflows.
Learn moreKAPA PROBE FAST qPCR Kits
Optimized for versatility and speed, and compatible with all probe-based SNP detection applications, KAPA PROBE FAST qPCR Kits provide fast and reproducible results for qPCR experiments using sequence-specific fluorogenic probe chemistries.
Learn moreKAPA HRM FAST qPCR Kits
Engineered to maximize differences in melting behavior between sequence variants, KAPA HRM FAST qPCR Kits yield accurate SNP genotyping with high sensitivity and speed.
Learn more