KAPA HyperPETE (Primer Extension Target Enrichment) is a novel hybridization capture technology designed to employ primer extension reactions to specifically capture and release target library molecules for sequencing.
This target enrichment technology preserves the performance of conventional hybridization while enabling a more efficient workflow. It has been specifically designed and optimized for small design sizes and validated to detect all major somatic variants in cfDNA, FFPE, and RNA samples, including SNVs, short indels, CNVs, MSI, and fusion transcripts (novel and known) and is especially suited for small panel oncology research applications.
The KAPA HyperPETE Portfolio includes readily available fixed-design panels for hereditary oncology, oncology hotspots, pan-cancer variants (with an MSI module), and lung cancer fusion variants. In addition, custom panels can be designed using HyperDesign, our easy-to-use online design tool.
Figure 1. The turnaround time (TAT) for KAPA HyperPETE target enrichment is similar to the TAT for amplicon-based workflows.
(A) While most hybridization-based workflows take two days to complete, KAPA HyperPETE workflows can be completed in one day. (B) Differences in the TAT for various applications of KAPA HyperPETE are dependent on the library preparation kit used, as each kit requires slightly different completion times. However, once the libraries are created, the enrichment workflow is the same across applications.
KAPA HyperPETE Panel | FFPET | Plasma cfDNA | Human cell-line gDNA | High-quality human gDNA | High-quality human RNA | |||
---|---|---|---|---|---|---|---|---|
DNA | RNA | Blood | Fresh Tissue | Blood | Fresh Tissue | |||
KAPA HyperPETE Choice / Explore | ✓ | ✓ | ✓ | ✓ | ✓ | |||
KAPA HyperPETE Choice / Explore RNA | ✓ | ✓ | ✓ | |||||
KAPA HyperPETE Pan Cancer Panel | ✓ | ✓ | ✓ | ✓ | ✓ | |||
KAPA HyperPETE Hot Spot Panel | ✓ | ✓ | ✓ | ✓ | ✓ | |||
KAPA HyperPETE Hereditary Oncology Panel | ✓ | ✓ | ✓ | |||||
KAPA HyperPETE Lung Cancer Fusion Panel | ✓ | ✓ | ✓ |
Leverage over a decade of probe-design experience with KAPA HyperPETE fixed design panels. When ordered, these readily available designs are delivered the next business day.
Contact us to request Panel Gene List, Data Data Proof of Principle, or Design Files.
The KAPA HyperPETE Pan Cancer Panel is a 302 Kb capture target panel covering 1324 target regions, 86 cancer related genes and 190 MSI loci relevant to somatic oncology research applications.The panel’s been optimized to deliver high uniformity and specificity from low quality, low quantity FFPET DNA and cfDNA samples.
The KAPA HyperPETE Hot Spot Panel is a 40 Kb capture target panel covering 37 targets from the genomic 'hot spot' regions that are frequently mutated in human cancer genes. It is optimized to deliver high uniformity and specificity from low quality, low quantity FFPET DNA and cfDNA samples.
The KAPA HyperPETE Hereditary Oncology Panel is a 200 Kb capture target panel focusing on hereditary breast and ovarian cancer research (including BRCA1 and BRCA2) as well as hereditary colorectal cancer research. It covers 47 related genes in these cancer types and has been optimized to deliver high uniformity and specificity in germline variant research with the pre-capture multiplexing capacity of 8 samples.
The KAPA HyperPETE Lung Cancer Fusion Panel is a 18 Kb capture target panel also using the novel KAPA HyperPETE technology to detect the known fusions and unknown fusion partners including 17 lung cancer fusion genes and 4 housekeeping genes (as internal controls).
The design and analysis (supported by NAVIFY® Mutation Caller) covers fusions, but the design also covers other variants near the capture region through alternative analysis solutions.
Use HyperDesign, a user-friendly online tool, that builds on two decades of in silico design experience to select probe panels that achieve deeper, more uniform downstream sequencing coverage with fewer sequencing reads—even across difficult-to-capture regions.
KAPA HyperPETE Choice | designed by customers using HyperDesign |
KAPA HyperPETE Explore | designed by the Roche Expert Designer Service |