KAPA HyperPETE

KAPA HyperPETE (Primer Extension Target Enrichment) is a novel hybridization capture technology designed to employ primer extension reactions to specifically capture and release target library molecules for sequencing. 

This target enrichment technology preserves the performance of conventional hybridization while enabling a more efficient workflow.  It has been specifically designed and optimized for small design sizes and validated to detect all major somatic variants in cfDNA, FFPE, and RNA samples, including SNVs, short indels, CNVs, MSI, and fusion transcripts (novel and known) and is especially suited for small panel oncology research applications. 

The KAPA HyperPETE Portfolio includes readily available fixed-design panels for hereditary oncology, oncology hotspots, pan-cancer variants (with an MSI module), and lung cancer fusion variants. In addition, custom panels can be designed using HyperDesign, our easy-to-use online design tool.

• Take hours off of total workflow time compared to typical hybridization capture, with time requirements similar to anchored multiplex methods (Figure 1)
• Enrich for long contiguous regions with fewer tubes per sample compared to amplicon technologies

Figure 1. The turnaround time (TAT) for KAPA HyperPETE target enrichment is similar to the TAT for amplicon-based workflows.

(A) While most hybridization-based workflows take two days to complete, KAPA HyperPETE workflows can be completed in one day. (B) Differences in the TAT for various applications of KAPA HyperPETE are dependent on the library preparation kit used, as each kit requires slightly different completion times. However, once the libraries are created, the enrichment workflow is the same across applications.

• Obtain more uniform coverage compared to anchored multiplex PCR (Figure 2)
• Avoid over- or under-representation of PCR-based target regions; KAPA HyperPETE technology uses primers to capture the regions of interest, rather than amplify them, greatly reducing amplification bias
• Streamline bioinformatics pipelines and increase your sequencing efficiency by eliminating the need to remove primer binding site sequences from your sequencing data