Ensuring quality in next generation sequencing results
A major challenge in Next-Generation Sequencing (NGS) is the ability to process low-input samples of variable quality with predictable success rates. Being able to determine the probability of sequencing success prior to library construction reduces sample processing time and cost.
Existing quantification methods using spectrophotometry or fluorimetry are typically insufficient to predict library yields and quality for degraded DNA samples.
Roche Sequencing Solutions offers a qPCR-based quantification and quality control kit for use with human genomic DNA samples. It combines the high performance of the KAPA SYBR FAST qPCR Master Mix with high-quality standards and allows for reliable assessment of concentration and quality with a single assay. The information gathered from this assay may be used to establish quality control minimums and optimize library construction parameters.