KAPA SYBR FAST

Overview

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.*

Benefits of KAPA SYBR FAST qPCR Kits

Optimal performance in stringent real-time PCR reaction conditions
Improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity compared to other commercial kits*

Refer to Instrument Compatibility Chart for guidance on compatible platforms.

Product Highlights

Quantitate changes in gene expression more accurately

High reaction efficiency between 95 – 105%, improving accuracy and reproducibility
Unbiased efficiency across a wide range of GC contents and amplicon lengths

View performance data showing reaction efficiency of 95 – 105% against amplicon length and GC-content using KAPA SYBR FAST qPCR Kits.

Detect low copy and difficult targets consistently

Improved processivity results in earlier Cq scores
Higher fluorescence detection across varying AT- and GC-rich targets

View data showing more accurate expression quantitation of difficult targets such as DMD (with 23.1% GC content) and NOTCH (with 72.2% GC content) by KAPA SYBR FAST qPCR Kit compared to other commercial kits.

Complete real-time PCR runs in just 40 minutes

55% shorter run times with fast cycling protocol
High performance when switching from standard to fast protocols

*Data on file.

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.