1. Roche Sequencing
  2. PCR
  3. KAPA Long Range PCR Kits

KAPA Long Range PCR Kits

Overview

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possesses 3–4X higher fidelity than Taq polymerase.

Features and Benefits of KAPA Long Range PCR Kits

  • Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step 
  •  Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
  •  Supplied in a 2X ReadyMix format with a loading dye, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye
  •  Contain all the components required for PCR except primers and template

Product Highlights

Amplification of long targets with high sensitivity

  • High sensitivity and specificity up to 15 kb
  • High yields with low input DNA

View data showing highly specific amplification of targets with different amplicon lengths using KAPA Long Range PCR kits.

Superior PCR performance with complex targets compared to competitor kits

  • Outperforms competitor kits in terms of high yield, sensitivity and specificity* 

View data showing greater sensitivity and specificity using KAPA Long Range PCR kits compared to competitor kits.

*Data on file.

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche.