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KAPA Pure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA and RNA next-generation sequencing (NGS) library construction workflows.
Features and Benefits:
High recovery of single- and double-stranded DNA (1 ng – 5 μg) in a single cleanup
Fast and efficient cleanups to remove unwanted reaction components
Figure 3. KAPA Pure Beads provides comparable performance to Agencourt AMPure XP in both DNA and RNA workflows.
Libraries were prepared with the KAPA HyperPlus Kit, from 100 ng high-quality E. coli genomic DNA, fragmented at 37˚C for 30 min to target a final library size of 300 bp.
Figure 4. KAPA Pure Beads provides comparable performance to Agencourt AMPure XP in both DNA and RNA workflows.
Libraries were prepared with the KAPA HyperPlus Kit, from 100 ng high-quality E. coli genomic DNA, fragmented at 37˚C for 30 min to target a final library size of 300 bp.
Figure 5. KAPA Pure Beads provides comparable performance to Agencourt AMPure XP in both DNA and RNA workflows.
Libraries were prepared with the KAPA Stranded RNA-Seq Kit with RiboErase, from 100 ng of Universal Human Reference (UHR) RNA according to standard protocol. Yields were measured with the KAPA Library Quantification Kit post ligation. Electropherograms for all libraries were generated with a Bioanalyzer 2100 High Sensitivity DNA Kit.
Figure 6. KAPA Pure Beads provides comparable performance to Agencourt AMPure XP in both DNA and RNA workflows.
Libraries were prepared with the KAPA Stranded RNA-Seq Kit with RiboErase, from 100 ng of Universal Human Reference (UHR) RNA according to standard protocol. Yields were measured with the KAPA Library Quantification Kit post ligation. Electropherograms for all libraries were generated with a Bioanalyzer 2100 High Sensitivity DNA Kit.
Flexible implementation at various points during library construction
Adjustable size selection parameters to achieve desired library sizes
View data showing tunable final library size distribution using KAPA Pure Beads compared to industry-leading supplier, and data on adjustable size selection at various points in library preparation using KAPA Pure Beads.
Figure 7. Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA HyperPrep Kit, from 1 μg high-quality E. coli genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Size selection (0.6X – 0.8X) using either KAPA Pure Beads (Figure 7-green) or Agencourt AMPure XP (Figure 8-blue) was performed after library amplification (2 cycles). Electropherograms were generated with a PerkinElmer LabChip GX DNA High Sensitivity Assay.
Figure 8. Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA HyperPrep Kit, from 1 μg high-quality E. coli genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Size selection (0.6X – 0.8X) using either KAPA Pure Beads (Figure 7-green) or Agencourt AMPure XP (Figure 8-blue) was performed after library amplification (2 cycles). Electropherograms were generated with a PerkinElmer LabChip GX DNA High Sensitivity Assay.
Figure 9: Adjustable size selection at various points in library preparation. Equivalent final library size is achieved by performing size selection either immediately after genomic DNA fragmentation or after adapter-ligation (Figure 9). Various final library sizes are achieved by tuning size selection parameters after adapter-ligation (Figure 10). All libraries were prepared with the KAPA HyperPrep Kit, from 100 ng high-quality human genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Electropherograms were generated with an Agilent 2100 Bioanalyzer High Sensitivity DNA Kit.
Figure 10. Adjustable size selection at various points in library preparation. Equivalent final library size is achieved by performing size selection either immediately after genomic DNA fragmentation or after adapter-ligation (Figure 9). Various final library sizes are achieved by tuning size selection parameters after adapter-ligation (Figure 10). All libraries were prepared with the KAPA HyperPrep Kit, from 100 ng high-quality human genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Electropherograms were generated with an Agilent 2100 Bioanalyzer High Sensitivity DNA Kit.
