KAPA Library Amplification Kits

Overview

KAPA Library Amplification Kits include KAPA HiFi DNA Polymerase, a novel enzyme engineered using our directed evolution technology for ultra-high fidelity and robustness. KAPA HiFi has become the enzyme of choice for next-generation sequencing (NGS) library amphficat1on due to its ability to amplify complex DNA populations with high fidelity, high efficiency and very low amplfication bias. This results in lower duplication rates and improved coverage of GC- and AT-nch regions, promoters, low-complexity and other challenging regions.

Benefits of KAPA Library Amplification Kits

  • Higher and more uniform coverage with lower dropout of difficult regions
  • Higher yields, low duplication rates and fewer wasted sequencing reads
  • Higher success rates with different sample types/applications
  • Convenience with the consistency of one core enzyme
  • Trusted as shown through over 10 years in the industry as well as thousands of peer-reviewed publications

Product Highlights

Reduced bias and efficient amplification of GC- and AT-rich genomes

  • Improved representation of all library fragments and sequence regions
  • Fewer cycles to achieve equivalent yields due to higher amplification efficiency

Improved sequencing coverage

  • Improved coverage uniformity of GC- and AT-rich regions, promoters, and other challenging regions
  • Increased coverage depth of difficult regions in targeted capture workflows, where two amplification steps are performed

Amplification of NGS libraries with ultra-high fidelity

  • Enhanced proofreading (3′-5′ exonuclease) activity using KAPA HiFi enzyme
  • Ultra-high fidelity for NGS applications

*Data on file.