Store kits for 18 months at -20°C.

Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgCl2 (25 mM). Both buffers contain MgCl2 at a final concentration of 2 mM.

KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix (2X), which contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM).

  KAPA HiFi PCR Kit   KAPA HiFi HotStart PCR Kit KAPA HiFI HotStart ReadyMix PCR Kit KAPA HiFi HotStart Library Amplification Kit KAPA HiFi HotStart Library Amplification Kit with Primer Mix KAPA HiFi HotStart Real-Time Library Amplification Kit
HotStart Enzyme No Yes Yes Yes Yes Yes
Format Components Components ReadyMix ReadyMix ReadyMix with Primer Mix ReadyMix
Kit Contents KAPA HiFi enzyme
KAPA HiFi buffers
Magnesium Chloride
dNTPs
KAPA HiFi HotStart enzyme
KAPA HiFi buffers
Magnesium Chloride
dNTPs
KAPA HiFi HotStart ReadyMix KAPA HiFi HotStart ReadyMix
KAPA HiFi HotStart ReadyMix
Amplification Primer Mix
KAPA HiFi HotStart ReadyMix
Fluorescein Standards
Recommended
Applications
PCR applications requiring flexibility and/or optimization PCR applications requiring a HotStart enzyme and flexibility and/or optimization Routine use in established PCR and NGS assays Routine NGS library amplification on any platform using user-supplied primers Routine NGS library amplification for Illumina® platforms NGS library amplification for Illumina® platforms that require careful control