KAPA HiFi Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA HiFi Kits
Overview

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high-fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in signifcant improvements to yield, sensitivity, speed, target length, and the ability to amplify diffcult templates (e.g., AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.

Product highlights

Achieve the highest fidelity
  • Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy
  • The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion

 

Comparison chart of KAPA HiFi vs. other products
Improve performance on GC- and AT-rich templates
  • Achieve higher success rates across targets up to 84% GC content
  • Higher yields when amplifying AT-rich templates
chart showing improved performance on GC- and AT-rich templates
Amplify longer targets with greater sensitivity
  • High-fidelity PCR of long and complex genomic templates
  • Improved sensitivity
  • High speed allows significantly shorter reaction times for long range PCR

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*Data on file.

Store kits for 18 months at -20°C.

Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgCl2 (25 mM). Both buffers contain MgCl2 at a final concentration of 2 mM.

KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix (2X), which contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM).

  KAPA HiFi PCR Kit KAPA HiFi HotStart PCR Kit KAPA HiFi HotStart Uracil+ Kit KAPA HiFI HotStart ReadyMix PCR Kit KAPA HiFi HotStart Library Amplification Kit KAPA HiFi HotStart Library Amplification Kit with Primer Mix KAPA HiFi HotStart Real-Time Library Amplification Kit
HotStart Enzyme No Yes Yes Yes Yes Yes Yes
Format MasterMix MasterMix ReadyMix ReadyMix ReadyMix ReadyMix with Primer Mix MasterMix
Kit Contents KAPA HiFi enzyme
KAPA HiFi buffer
KAPA Hifi GC buffer
Magnesium Chloride dNTPs
KAPA HiFi HotStart enzyme
KAPA HiFi buffer
KAPA HiFi GC buffer
Magnesium Chloride
dNTPs
KAPA HiFi HotStart ReadyMix, MgCl2 KAPA HiFi HotStart ReadyMix KAPA HiFi HotStart ReadyMix

 

KAPA HiFi HotStart ReadyMix
Amplification Primer Mix
KAPA HiFi HotStart ReadyMix
Fluorescent Standards
Recommended Applications Includes buffers for high-fidelity PCR and difficult targets, including GC-rich templates Antibody-mediated hot start with buffers for high-fidelity PCR and difficult targets, including GC-rich templates Antibody-mediated hot start PCR for bisulfite sequencing Antibody-mediated hot start in a convenient ReadyMix formulation for routine high-fidelity PCR Antibody-mediated hot start for routine high-fidelity library amplification with user-supplied sequencing primers Antibody-mediated hot start for routine high-fidelity library amplification with Illumina adapters and indexing primers Replace post-amplification QC by gel electrophoresis with real-time monitoring of amplification against a target range

For purchasing or general questions

For online ordering

KAPA HiFi PCR Kits

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KAPA HiFi PCR Kits

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK2101
07958838001
With dNTPs 100 U
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KK2102
07958846001
With dNTPs 250 U
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KK2501
07958889001
HotStart PCR Kit, with dNTPs 100 U
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KK2502
07958897001
HotStart PCR Kit, with dNTPs 250 U
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KK2601
07958927001
HotStart ReadyMix, 100 x 25 µL reactions 1.25 mL
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KK2602
07958935001
HotStart ReadyMix, 500 x 25 µL reactions 6.25 mL
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09420398001
09420398001

HiFi HotStart ReadyMix, 9.6ml

9.6 mL
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KAPA HiFi Kits for bisulfite sequencing

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KAPA HiFi Kits for bisulfite sequencing

KAPA CODE ROCHE CAT NO DESCRIPTION KIT SIZE
KK2801 7959052001 KAPA HiFi HotStart Uracil+ Kit 50 rxns
KK2802 7959079001 KAPA HiFi HotStart Uracil+ Kit 250 rxns

KAPA HiFi Library Amplification Kits

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KAPA HiFi Library Amplification Kits

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK2611
07958951001
HotStart Ready Mix without primer mix 50 x 50 µL reactions
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KK2612
07958960001
HotStart Ready Mix without primer mix 250 x 50 µL reactions
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KK2620
07958978001
HotStart ReadyMix with primer mix 50 x 50 µL reactions
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KK2621
07958986001
HotStart ReadyMix with primer mix 250 x 50 µL reactions
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KK2702
07959028001
HotStart ReadyMix real-time PCR library amplification, with fluorescent standards 250 x 50 µL reactions
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For purchasing or general questions

For online ordering