Digital LightCycler® dPCR System

Unleash the true power of digital PCR
Image of the Digital LightCycler dPCR System, including the partitioning engine, analyzer, and partitioning plates

Make the leap from idea to impact

It's time for a leap forward in digital PCR technology. 

 

The Roche Digital LightCycler® System is the digital PCR instrument of tomorrow. With a unique combination of 3 nanowell plate configurations, 6 advanced optical channels, and 5x concentrated DNA and RNA master mixes, it has the potential to help your lab to make the leap from publishing research to producing clinically viable assays.

The unique combination with the potential to change the future of digital PCR

Image of the Digital LightCycler dPCR System, including the partitioning engine, analyzer, and partitioning plates

Bespoke testing with 3 one-of-a-kind1-4 nanowell plates

The unique hexagonal-shaped nanowell partitions and the filling dye that validates partitioning gives the Digital LightCycler® System the ability to handle a wide variety of extracted samples such as FFPET, cfDNA, Whole Blood, and more.

Through these partitions, target sequences are efficiently concentrated within isolated microreactors. This concentration effect reduces template competition, thereby enabling the detection of rare mutations and allowing for a higher tolerance to inhibitors present in a sample.

Diagram showing hexagonal well pattern on the High Resolution Plate (100,000 partitions)
High Resolution


15μL, 100,000 partitions

Copy Number Variation

Human Genetic Disease

Diagram showing hexagonal well pattern on the Universal Plate (28,000 partitions)
Universal Plate


30μL, 28,000 partitions

Gene Expression

Transplant Rejection

Diagram showing hexagonal well pattern on the High Sensitivity Plate (20,000 partitions)
High Sensitivity


45μL, 20,000 partitions

Cell-free DNA

Oncology

Rare Mutation Detection

Photo of a man examining data on computer screen
Flexible 6-channel multiplexing*

6 advanced optical channels enable a high degree of multiplexing for DNA or RNA targets.

  • Batch size increments of between 8 and 96 samples per run
  • Demonstrated with both DNA and RNA reagents
  • Analyzer run times vary for different batch sizes to save time on smaller batches
High sample utilization with 5x concentrated concentrated DNA and RNA master mixes*

Get the most out of your samples with the Digital LightCycler® System's 5x concentrated master mixes.

  • High sample utilization in each reaction means confidence in results
  • 1 Step RNA (RT) with a hold time of just 15 minutes
  • DNA and RNA optimized for multiplexing
  • Filling dye control that validates partitioning
  • Single tube
  • 50-75% less primer/probe usage

* Roche data on file: DH_02365.01_031B_Digital_LightCycler_Reagent_Feasibility_Report_v3,
Document Number: 0000000000001200000501942

Roche data on file: DH-02365.01-500E_Digital LightCycler System Performance report

Roche data on file: DHF Digital_LightCycler_Reagent_Feasibility_Report

Roche data on file: DH-02365.01-008B_Digital_LightCycler_System_Feasibility_Report

Bar chart showing that the Digital LightCycler enables the use of relatively large volumes of sample vs. another dPCR system

Sample utilization vs. competitive system1-4

Simple workflow and data tracking

Icon showing a pipette transfering sample between tubes
  1. First batches are created either manually or with orders from the Laboratory Information System (LIS). The system automatically sets up the layout and creates the samples.
Icon of a microfuge tube
  1. The newly extracted samples are then transferred to the PCR where a pipette is used to mix the samples and the master mix. The nanowell plate of choice is scanned into the web application for full sample tracking.
icon of a pipette and a dPCR partitioning plate
  1. The PCR mix is then transferred into the nanowell plate which is then loaded into the partitioning engine. This is done for each plate.
Icon of a stack of partitioning plates
  1. All the plates are then loaded into the Analyzer.
Icon of a laptop computer showing internet signal
  1. After the run is complete, positives and negatives are counted up and the Poisson calculation is applied.
Icon of a stack of discs
  1. Results are validated and then sent back to the LIS.

References

  1. https://www.qiagen.com/us/products/instruments-and-automation/pcr-instruments/qiacuity-digital-pcr-system/ (last accessed on 06/17/2022)
  2. https://www.bio-rad.com/en-us/life-science/digital-pcr/qx-one-droplet-digital-pcr-ddpcr-system (last accessed on 06/17/2022)
  3. https://www.stillatechnologies.com/6-color-dpcr/ (last accessed on 06/17/2022)
  4. https://www.thermofisher.com/order/catalog/product/4489084 (last accessed on 06/17/2022)

It’s time for a leap forward in digital PCR technology

Experience sensitivity, precision, flexibility, and integration in one powerful
clinical research tool. The Digital LightCycler® System from Roche is a
digital PCR system that can help laboratories push forward the boundaries
of clinical research and has the potential to advance global medical knowledge.  

Learn more in our brochures

View the Tech Spotlights

The digital PCR Principle

Digital PCR (dPCR) is a relatively new PCR-based method for quantification of DNA and RNA targets. It builds on traditional PCR amplification methods to provide precise, absolute quantification of nucleic acids with single molecular resolution.

Key advantages of digital PCR:

  • Absolute quantification without need for references standards
  • Increased detection sensitivity for low-abundance targets due to enrichment
  • Delivers high precision (low CV) over a wide dynamic range
  • More partitions deliver even high precision 

Digital LightCycler Evaluator Data Overview

Digital PCR applications for oncology research: methylation analysis and variant detection

This presentation from AACR 2024 highlights two different oncology research applications for Roche’s Digital LightCycler® dPCR System. First, Yu Zhao, Roche Sr. Applications Specialist, describes the DNA methylation analysis of the RASSF1A promoter in cancer cell lines; this assay enables precise detection and absolute quantification without requiring bisulfite conversion or reference standards. Then, GT Molecular discusses a new AML research panel developed for the Digital LightCycler®; this assay enables sensitive detection of AML-associated variants using DNA extracted from liquid biopsy samples. The assay highlights and leverages the clinical utility of the Digital LightCycler® dPCR System.

Digital LightCycler Overview

It is time to take a leap forward in digital PCR technology with Roche’s Digital LightCycler® System

Presenter: Simran Saini, PhD

Principal Scientist II, Sequencing Workflows, Applications and Technology, Roche

In this presentation, we discuss the Digital LightCycler® System, a digital system for DNA and RNA with 6-color capability for flexible, multiplexed biomarker detection. With advances in sensitivity, precision, flexibility, and integration, the Digital LightCycler® System is a powerful digital PCR system that aims to help laboratories push the boundaries of clinical research forward. Three configurations of consumables are available to meet varied application needs, including high sample volume for cfDNA screening or greater number of partitions for high-resolution CNV analysis. We present data that demonstrates the sensitive performance of the Digital LightCycler® System as the powerful new addition to the Roche PCR ecosystem.

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Roche Cat. No
 Description
09274804001 Digital LightCycler® Analyzer
09224777001 Digital LightCycler® Partioning Engine
09393544001 Digital LightCycler® DNA Master Kit
08897115001 Digital LightCycler® RNA Master Kit
09033696001 Digital LightCycler® Universal Plate
09033700001 Digital LightCycler® HiRes Plate
09033718001 Digital LightCycler® HiSens Plate
08897115001 Digital LightCycler® Partioning Fluid
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Roche Cat. No
 Description
10068469001 LightMix® Digital EGFR_Ex19del
10068434001 LightMix® Digital EGFRT790M
10068540001 LightMix® Digital BRAFV600E
10068531001 LightMix® Digital KRASG12D
10068507001 LightMix® Digital PIK3CAE535K

LightMix® Digital Assays are developed and manufactured by TIB MOLBIOL GmbH, Berlin, Germany and exclusively distributed by Roche.

For Life Science Research Only.

Not For Use in Diagnostic Procedures. LIGHTMIX is a trademark of TIB MOLBIOL.

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