Purchase convenient all-in-one kits or simply the KAPA HyperExome Probes in convenient reaction pack sizes, which start from 12 reactions and exceed 4000 reactions.
The KAPA HyperExome Probes are Roche’s brand new Whole Exome Sequencing solution, based on the next generation KAPA Target Enrichment Probe’s technology. Their design and technology provide broad, relevant database coverage and high sequencing efficiency.
The product has been extensively optimized through design and manufacturing processes to increase coverage in hard-to-sequence regions for a more uniform and complete exome. Based on customer feedback, Roche Sequencing scientists created the KAPA HyperExome Probes, a sequencing efficient design with actionable and well-annotated content.
KAPA HyperExome Probes are targeting the GRCh38/hg38 genome assembly (coordinates for hg19 annotation are also provided) by covering the CCDS, RefSeq, Ensembl, GENCODE and ClinVar genomic databases in an efficient ~43 Mb capture target size, including 387 sample tracking SNPs to streamline sample identity tracking throughout the WES workflow.
Figure 1. Database data retrieved in Feb 2020, unpadded capture target used to compare across vendors.
Figure 2. Normalized capture coverage per GC 5% bins. KAPA HyperExome Probes showing more optimal use of sequencing by minimizing regions that are less covered or regions getting more reads than really needed. KAPA HyperExome Probes (capture target 43 Mb) sequenced at 60 M HQ reads and supplier X at 51.2 M HQ reads, proportional to its smaller capture target (36.7 Mb).
Figure 3. KAPA HyperExome Probes showing higher % of reads on-target that together with higher uniformity deliver deeper median coverage and increasingly better target coverage at 30x, 40x or 50x. KAPA HyperExome Probes (capture target 43 Mb) sequenced at 60 M HQ reads and supplier X at 51.2 M HQ reads, proportional to its smaller capture target (36.7 Mb).
Figure 4. KAPA HyperExome Probes shows better uniformity by a lower Fold 80 base penalty than Supplier X’s Exome. (Fold 80 shows the fold amount of additional sequencing to bring 80% of the target to the current mean coverage, lower is better).
The design and annotation files provide information about genomic regions covered by the capture probes and the genes included in these regions.
The KAPA HyperExome Probes were designed to cover coding exons sequence from the following annotation sources: CCDS release 20, RefSeq (July 30, 2018), Ensembl release 93, and GENCODE release 28. It is also covering ClinVar variants (pathogenic, likely pathogenic, variants of unknown significance – as of July 29, 2018), the TERT promoter and 387 sample tracking SNPs. The genome assembly coordinates are based on the UCSC human genome build hg38, though hg19 files are also provided.
The total design size is 43 Mb of capture target space; the capture targets BED file shows the capture probe footprint. The UCSC Browser BED files are provided for upload as a custom track. All design files are provided in the original hg38 genome assembly and also converted to hg19 genome assembly. Conversion was performed using the NCBI Genome Remapping Service.
Demo data is available for evaluation. Please contact your local Roche representative.
Purchase convenient all-in-one kits or simply the KAPA HyperExome Probes in convenient reaction pack sizes, which start from 12 reactions and exceed 4000 reactions.
| Roche Cat. No | Description | Kit Size (reactions) |
|---|---|---|
| 09107592001 | KAPA HyperExome Prep Kit - 192 samples | Convenient all-in-one package for 192 samples – all kits provided except the KAPA UDI Primer Mixes – For Covaris sonicated DNA input - Talk to your Roche representative today |
| 09107606001 | KAPA HyperExome Plus Kit - 192 samples | Convenient all-in-one package for 192 samples – all kits provided except the KAPA UDI Primer Mixes – Integrated enzymatic DNA shearing - Talk to your Roche representative today |
| 09062548001 | KAPA HyperExome, 12 rxn | 12 Reactions |
| 09062556001 | KAPA HyperExome, 24 rxn | 24 Reactions |
| 09062564001 | KAPA HyperExome, 48 rxn | 48 Reactions |
| 09062572001 | KAPA HyperExome, 96 rxn | 96 Reactions |
| 09062599001 | KAPA HyperExome, 192 rxn | 192 Reactions |
| 09062602001 | KAPA HyperExome, 384 rxn | 384 Reactions |
| 09062629001 | KAPA HyperExome, 768 rxn | 768 Reactions |
| 09062637001 | KAPA HyperExome, 1152 rxn | 1152 Reactions |
| 09062645001 | KAPA HyperExome, 1536 rxn | 1536 Reactions |
| 09062653001 | KAPA HyperExome, 4000 rxn | 4000 Reactions (made to order) |
| 09062661001 | KAPA HyperExome, 10000 rxn | 10000 Reactions (made to order) |
| 09062670001 | KAPA HyperExome, 20000 rxn | 20000 Reactions (made to order) |
| 09062688001 | KAPA HyperExome, 50000 rxn | 50000 Reactions (made to order) |
| 09062696001 | KAPA HyperExome, 100000 rxn | 100000 Reactions (made to order) |