KAPA HyperExome Probes

For Research Use Only. Not for use in diagnostic procedures.

KAPA HyperExome product


The KAPA HyperExome Probes are Roche’s brand new Whole Exome Sequencing solution, based on the next generation KAPA Target Enrichment Probe’s technology. Their design and technology provide broad, relevant database coverage and high sequencing efficiency.

The product has been extensively optimized through design and manufacturing processes to increase coverage in hard-to-sequence regions for a more uniform and complete exome. Based on customer feedback, Roche Sequencing scientists created the KAPA HyperExome Probes, a sequencing efficient design with actionable and well-annotated content.

KAPA HyperExome Probes are targeting the GRCh38/hg38 genome assembly (coordinates for hg19 annotation are also provided) by covering the CCDS, RefSeq, Ensembl, GENCODE and ClinVar genomic databases in an efficient ~43 Mb capture target size, including 387 sample tracking SNPs to streamline sample identity tracking throughout the WES workflow.


Features and benefits of KAPA HyperExome Probes

  • Better by design - Uncover difficult regions and access more content from important genomic databases
  • Validated with the KAPA HyperCap Workflow v3.0 for high sequencing efficiency by superior uniformity and low duplication rates
  • Higher result confidence with >98.7% sensitivity and >99.7% specificity of SNP detection
  • Stronger enrichment by high fidelity probes that are manufactured with KAPA HiFi DNA Polymerase and target both DNA strands
  • Streamline your sample identity tracking to new levels of result confidence by intrinsically targeting 387 sample tracking SNPs 
  • Experience consistent quality and efficiency with NGS probe pool QC and larger reaction pack sizes

Performance data

Better by design for higher result confidence
  • Utilize a whole exome solution with exceptional exon coverage of genes and variants from CCDS, RefSeq, Ensembl, GENCODE, ClinVar for maximal coverage of regions of medical research relevance in an efficient capture target size of just 43 Mb
  • Achieve better sequencing coverage of the important medical research content for less repeats and higher result confidence
High performing KAPA HyperExome Probes for superior uniformity and sequencing efficiency
  • Obtain uniform coverage across the GC% range for better sequencing efficiency
  • Experience the finest balance of high on-target rates with high uniformity for better and deeper target coverage
KAPA HyperExome Design Files

The design and annotation files provide information about genomic regions covered by the capture probes and the genes included in these regions.

The KAPA HyperExome Probes were designed to cover coding exons sequence from the following annotation sources: CCDS release 20, RefSeq (July 30, 2018), Ensembl release 93, and GENCODE release 28. It is also covering ClinVar variants (pathogenic, likely pathogenic, variants of unknown significance – as of July 29, 2018), the TERT promoter and 387 sample tracking SNPs. The genome assembly coordinates are based on the UCSC human genome build hg38, though hg19 files are also provided.

The total design size is 43 Mb of capture target space; the capture targets BED file shows the capture probe footprint. The UCSC Browser BED files are provided for upload as a custom track. All design files are provided in the original hg38 genome assembly and also converted to hg19 genome assembly. Conversion was performed using the NCBI Genome Remapping Service.

Demo data

Demo data is available for evaluation. Please contact your local Roche representative.

To place an order or ask a question
Contact us

Purchase convenient all-in-one kits or simply the KAPA HyperExome Probes in convenient reaction pack sizes, which start from 12 reactions and exceed 4000 reactions.

Roche Cat. No Description Kit Size (reactions)
09107592001 KAPA HyperExome Prep Kit - 192 samples Convenient all-in-one package for 192 samples – all kits provided except the KAPA UDI Primer Mixes – For Covaris sonicated DNA input - Talk to your Roche representative today
09107606001 KAPA HyperExome Plus Kit - 192 samples Convenient all-in-one package for 192 samples – all kits provided except the KAPA UDI Primer Mixes – Integrated enzymatic DNA shearing - Talk to your Roche representative today
09062548001 KAPA HyperExome, 12 rxn 12 Reactions
09062556001 KAPA HyperExome, 24 rxn 24 Reactions
09062564001 KAPA HyperExome, 48 rxn 48 Reactions
09062572001 KAPA HyperExome, 96 rxn 96 Reactions
09062599001 KAPA HyperExome, 192 rxn 192 Reactions
09062602001 KAPA HyperExome, 384 rxn 384 Reactions
09062629001 KAPA HyperExome, 768 rxn 768 Reactions
09062637001 KAPA HyperExome, 1152 rxn 1152 Reactions
09062645001 KAPA HyperExome, 1536 rxn 1536 Reactions
09062653001 KAPA HyperExome, 4000 rxn 4000 Reactions (made to order)
09062661001 KAPA HyperExome, 10000 rxn 10000 Reactions (made to order)
09062670001 KAPA HyperExome, 20000 rxn 20000 Reactions (made to order)
09062688001 KAPA HyperExome, 50000 rxn 50000 Reactions (made to order)
09062696001 KAPA HyperExome, 100000 rxn 100000 Reactions (made to order)
Talk with your Roche Sales Representative for additional pack sizes.

Reagents and accessories are also available separately.
To place an order or ask a question
Contact us