Figure 2. Variants from six RNA controls of SARS-CoV-2 are identified from within a single (contrived) sample. A total of 1,000,000 SARS-CoV-2 genome copies from 6 different viral RNA controls were combined with either 20 ng (blue) or 100 ng (green) of human universal reference RNA and processed in triplicate using KAPA RNA HyperPrep Kit and the KAPA HyperCap SARS-CoV-2 Target Enrichment Panel. Alternate allele frequency analysis shows that all expected variants from the six SARS-CoV-2 controls were identified at close to the expected frequency. Datasets downsampled to 1 million clusters (2 x 75 bp, duplicates retained for this analysis) prior to analysis.