We are witnessing one of the most fascinating discoveries in science as it unfolds. In 2012, a landmark paper was published by Doudna and colleagues,1 launching a then little-known terminology called CRISPR into the scientific space and propelling a revolution that could very well still be in its infancy. As scientists understand its enormous potential and attempt to overcome its limitations, CRISPR has since become a “lab-hold” name. Since the demonstration of how CRISPR can be used as a gene-editing tool in 2012, there has been a 15-fold increase in the number of publications involving this tool to date (PubMed). While the potential of CRISPR in gene editing has been shown in a variety of applications, the technique has also stirred up controversy. Nevertheless, the progress attained so far using CRISPR and the promise of it being used as a standard genetic engineering tool for gene therapy is simply undeniable. In this article, we’ll take you through the exciting CRISPR journey to see what this genetic tool is capable of achieving, the variety of applications it has had, its reach and penetrance, and review the benefits and ramifications of CRISPR research. CRISPR as a gene-editing tool is used in several applications and Roche products have been successfully integrated into some of them, such as human embryonic stem cell research and single-cell molecular screening. Roche products have also been useful in the identification of genome-wide off target cleavage sites of CRISPR/Cas9 using CRICLE-Seq. We’ll also discuss some of these applications.