KAPA HTP/LTP Library Preparation Kits


KAPA HTP/LTP Library Preparation Kits employ our first-generation “with bead” workflow for DNA library construction. These kits contain optimally formulated enzymes selected through our directed evolution technology and offer higher success rates than conventional library construction kits in both high- and low-throughput sample preparation pipelines. 

KAPA HTP/LTP Library Preparation Kits are compatible with most automation platforms and provide a complete library preparation solution together with KAPA Adapters and KAPA Pure Beads. 

For our more streamlined, highly automatable library preparation solution, we would recommend using the KAPA HyperPrep Kits and KAPA HyperPlus Kits. These workflows offer higher conversion of input into library molecules and therefore are particularly well suited for applications with lower inputs and challenging samples such as FFPE-derived DNA. 


Features and Benefits of KAPA HTP/LTP Library Preparation Kits

  • Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions
  • Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme 
  • Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification 
  • Qualified automation methods


Product Highlights

Greater molecular complexity

  • Higher adapter-ligated library yields, translating to higher library complexity
  • Fewer cycles of amplification needed, resulting in lower PCR duplication rates, which are critical for library construction from lower input and challenging samples

Reduced amplification bias and improved coverage

  • Reduced PCR bias* using KAPA HiFi enzyme
  • Improved coverage uniformity of across the entire range of genomic GC content

Automation with qualified scripts

  • Qualified methods available for major liquid handling platforms used in NGS, such as PerkinElmer Sciclone NGS and NGSx, Beckman Coulter Biomek FXp and FX dual-pod, Agilent Technologies NGS Workstation Option B, and Eppendorf® epMotion 5075 TMX

*Data on file.