KAPA HTP/LTP Library Preparation Kits employ our first-generation “with bead” workflow for DNA library construction. These kits contain optimally formulated enzymes selected through our directed evolution technology and offer higher success rates than conventional library construction kits in both high- and low-throughput sample preparation pipelines.
KAPA HTP/LTP Library Preparation Kits are compatible with most automation platforms and provide a complete library preparation solution together with KAPA Adapters and KAPA Pure Beads.
For our more streamlined, highly automatable library preparation solution, we would recommend using the KAPA HyperPrep Kits and KAPA HyperPlus Kits. These workflows offer higher conversion of input into library molecules and therefore are particularly well suited for applications with lower inputs and challenging samples such as FFPE-derived DNA.
Features and Benefits of KAPA HTP/LTP Library Preparation Kits
Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions
Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme
Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification
Qualified automation methods
Features and Benefits of KAPA HTP/LTP Library Preparation Kits
Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions
Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme
Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification
Qualified automation methods
Product Highlights
Greater molecular complexity
Higher adapter-ligated library yields, translating to higher library complexity
Fewer cycles of amplification needed, resulting in lower PCR duplication rates, which are critical for library construction from lower input and challenging samples
Improved coverage uniformity of across the entire range of genomic GC content
Automation with qualified scripts
Qualified methods available for major liquid handling platforms used in NGS, such as PerkinElmer Sciclone NGS and NGSx, Beckman Coulter Biomek FXp and FX dual-pod, Agilent Technologies NGS Workstation Option B, and Eppendorf® epMotion 5075 TMX