KAPA HyperPure Beads

Overview

KAPA HyperPure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA next-generation sequencing (NGS) library construction workflows.

Features and Benefits

High recovery of single- and double-stranded DNA (1 ng – 5 μg) in a single cleanup
Fast and efficient cleanups to remove unwanted reaction components
Easy substitution into bead-based workflows
Adjustable size selection
Automation friendly
Larger pack sizes for higher throughput

Product highlights

Seamless Integration into NGS Workflows

Compatible with all KAPA DNA library preparation protocols
Comparable yields and size-distribution in comparison to industry-leading supplier
Readily incorporated into existing automation applications

View data on comparable performance of KAPA HyperPure Beads on DNA post-ligation yield compared to industry-leading supplier.

Tunable and Highly Reproducible Size Selection

Reproducible size selection of nucleic acids - capture of fragmented input DNA, adapter-ligated molecules, or amplified libraries in NGS library preparation workflows.
Enables researchers to consistently select for fragment sizes of interest without bias.
Fast, efficient reaction cleanups remove adapters, adapter-dimers, primers, primer-dimers, nucleotides, salts, and enzymes.
KAPA HyperPure Beads are ready-to-use and automation-friendly.

KAPA HyperPure Beads provide tunable size selection of double-stranded DNA. At each step of the KAPA HyperPrep library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto Bioanalyzer. (A) Post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection. (B) Post-amplification size selection. Libraries were amplified, and final libraries were then subjected to size selection.

All graphic data is on file, unless otherwise noted.

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.