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KAPA HyperPure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA next-generation sequencing (NGS) library construction workflows.
View data on comparable performance of KAPA HyperPure Beads on DNA post-ligation yield compared to industry-leading supplier.
KAPA HyperPure Beads provide tunable size selection of double-stranded DNA. At each step of the KAPA HyperPrep library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto Bioanalyzer. (A) Post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection. (B) Post-amplification size selection. Libraries were amplified, and final libraries were then subjected to size selection.
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Research Use Only. Not for use in diagnostic procedures.
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