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KAPA HyperPure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA next-generation sequencing (NGS) library construction workflows.
Features and Benefits
High recovery of single- and double-stranded DNA (1 ng – 5 μg) in a single cleanup
Fast and efficient cleanups to remove unwanted reaction components
Figure 1. KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows. (A) Recovery of unfragmented human gDNA. (B) Recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA. Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup.
Figure 2. KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows. (A) Recovery of unfragmented human gDNA. (B) Recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA. Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup.
Figure 3. KAPA HyperPure Beads provide tunable size selection of double-stranded DNA. At each step of the KAPA HyperPrep library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto the Bioanalyzer. (A) Post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection. (B) Post-amplification size selection. Libraries were amplified and final libraries were then subjected to size selection.
Figure 4. KAPA HyperPure Beads provide tunable size selection of double-stranded DNA. At each step of the KAPA HyperPrep library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto the Bioanalyzer. (A) Post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection. (B) Post-amplification size selection. Libraries were amplified and final libraries were then subjected to size selection.
