KAPA Library Quantification Kits

Kits can be stored at -20˚C.

Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.

Specifications
Spec Description
Compatible Platforms Illumina HiSeq, NextSeq, MiSeq
Library Type
Any
Starting Material
Any NGS library ready for cluster amplification or emPCR
Standard curve concentration range
20–0.0002 pM for Illumina libraries
Sequencing Applications
Whole Genome Sequencing, Whole Exome Sequencing, Targeted Sequencing (custom panels), RNA-Seq, ChIP-Seq, Amplicon Sequencing, Methyl-Seq
Components–Illumina Platform
  Kit code Roche Cat. No.

10X Illumina

Primer Premix

2X KAPA  

SYBR FAST qPCR

Master Mix

(universal)

50X KAPA

SYBR FAST ROX High

50X KAPA

SYBR FAST ROX Low

2X KAPA

SYBR FAST qPCR

Master Mix

(with equipment-specific dye)

2X KAPA

SYBR FAST

qPCR Master Mix

(ROX Low)

Illumina DNA Standards 1-6
KAPA Library Quantification Kits - Complete Kit (universal)
KK4824 07960140001 X X X X     X
KAPA Library Quantification Kits - Complete Kit (with equipment-specific dye)

KK4835

KK4844

KK4854

07960204001

07960255001

07960298001

X       X   X
KAPA Library Quantification Kits - Complete Kit (ROX Low)

KK4873

 

07960336001 X         X X
KAPA Library Quantification Kits - qPCR MasterMix (universal) and Primer Premix only KK4923 07960441001 X X X X      

KAPA Library Quantification Kits - qPCR MasterMix (with equipment-specific  reference dye)

 and Primer Premix only

KK4933

KK4943

KK4953

07960484001

07960522001

07960573001

X       X    
KAPA Library Quantification Kits - qPCR MasterMix (ROX Low)
and Primer Premix only
KK4973 07960727001 X         X