Primer Extension-Based Target Enrichment

Overview

Based on primer extension technology, KAPA HyperPETE technology provides a single workday workflow for target enrichment. The integrated end-to-end offering makes for more efficient and effective processes, enabling you to shift your focus to what matters most. Providing both catalog or custom panels with an integrated analysis solution (NAVIFY^® Mutation Caller), KAPA HyperPETE Workflow is especially suited for small panel oncology research applications.

Benefits of the KAPA HyperPETE Workflow

High uniformity for better sequencing efficiency with less optimization
Single day workflow by shorter incubations and reduced hands-on-time
Custom design expertise enables access to difficult genomic regions

KAPA HyperPETE Catalog Panels

Suitable for small panel oncology research applications, KAPA HyperPETE catalog panels have been optimized for somatic oncology research workflows and provide precise variant calling for SNV, CNV, indels, fusions and MSI. The KAPA HyperPETE Workflow offers integrated secondary analysis provided by NAVIFY® Mutation Caller.

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KAPA HyperPETE Choice and KAPA HyperPETE Explore Panels

KAPA HyperPETE Choice and KAPA HyperPETE Explore Panels enable custom panel design up to 250Kb for DNA inputs and 50Kb for RNA inputs. Based on Roche’s renowned design and content expertise, our workflow is specifically optimized for small design sizes and validated to detect all major mutation classes. The KAPA HyperPETE Workflow offers integrated secondary analysis provided by NAVIFY® Mutation Caller.

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Workflow considerations & comparisons

Streamlining every step of the process from pipetting to purchasing, Roche Sample Prep Solutions encompass all the steps required to convert a sample to a sequencing-ready library. From sample collection to library quantification, we offer integrated workflow solutions for different sample types and sequencing applications that are proven, simple and complete.

	KAPA HyperPETE Workflow	KAPA HyperCap Workflow	Amplicon-based workflow
Ease of use	Easy to use and automate Get your answers faster, without compromising on performance Streamlined 1-day workflow with short incubations and reduced hands-on time	Easy to use and automate Streamlined and optimized workflow with low hands-on-time	Easy to use and automate Fastest workflow
Performance	Highest sequencing efficiency in small panels Detects all major mutation classes	High sequencing efficiency Detects all major mutation classes	Lower uniformity Lower performance in CNV detection Impossible to detect unknown fusion partners Good performance on SNVs / Indels and known fusions
Technology	Primer Extension Target Enrichment	Hybridization-based capture	PCR amplification
Turnaround time	Single day	1.5 days	Single shift
Panel size	Up to 250 Kb	Up to Whole Exome and beyond	Up to a few megabases
Applications	Somatic oncology research RNA fusions (known + novel fusion partners)	Hereditary genetics research Hereditary and somatic Oncology research	Somatic oncology research Hereditary genetics RNA fusions (Known only)
Secondary analysis support	Germline and somatic variants secondary analysis in NAVIFY Mutation Caller Germline and somatic variants secondary analysis with open source bioinformatic tools	Germline variants secondary analysis in NAVIFY Mutation Caller Germline and somatic variants secondary analysis with open source bioinformatic tools	Various vendor offerings

Research Use Only. Not for use in diagnostic procedures.

KAPA HYPERPETE, HYPERCAP, KAPA and NAVIFY are trademarks of Roche. All other product names and trademarks are the property of their respective owner.