Primer Extension-Based Target Enrichment

KAPA HyperPETE

Overview

Based on primer extension technology, KAPA HyperPETE technology provides a single workday workflow for target enrichment. The integrated end-to-end offering makes for more efficient and effective processes, enabling you to shift your focus to what matters most. Providing both catalog or custom panels with an integrated analysis solution (NAVIFY® Mutation Caller), KAPA HyperPETE Workflow is especially suited for small panel oncology research applications.

Benefits of the KAPA HyperPETE Workflow

  • High uniformity for better sequencing efficiency with less optimization

  • Single day workflow by shorter incubations and reduced hands-on-time

  • Custom design expertise enables access to difficult genomic regions

     

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KAPA HyperPETE Catalog Panels

Suitable for small panel oncology research applications, KAPA HyperPETE catalog panels have been optimized for somatic oncology research workflows and provide precise variant calling for SNV, CNV, indels, fusions and MSI. The KAPA HyperPETE Workflow offers integrated secondary analysis provided by NAVIFY® Mutation Caller.

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KAPA HyperPETE Choice and KAPA HyperPETE Explore Panels

KAPA HyperPETE Choice and KAPA HyperPETE Explore Panels enable custom panel design up to 250Kb for DNA inputs and 50Kb for RNA inputs. Based on Roche’s renowned design and content expertise, our workflow is specifically optimized for small design sizes and validated to detect all major mutation classes. The KAPA HyperPETE Workflow offers integrated secondary analysis provided by NAVIFY® Mutation Caller.

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Workflow considerations & comparisons

Streamlining every step of the process from pipetting to purchasing, Roche Sample Prep Solutions encompass all the steps required to convert a sample to a sequencing-ready library. From sample collection to library quantification, we offer integrated workflow solutions for different sample types and sequencing applications that are proven, simple and complete.

     

  KAPA HyperPETE Workflow KAPA HyperCap Workflow Amplicon-based workflow

Ease of use

  • Easy to use and automate
  • Get your answers faster, without compromising on performance
  • Streamlined 1-day workflow  with short incubations and reduced hands-on time
  • Easy to use and automate
  • Streamlined and optimized workflow with low hands-on-time
  • Easy to use and automate
  • Fastest workflow
Performance
  • Highest sequencing efficiency in small panels
  • Detects all major mutation classes
  • High sequencing efficiency
  • Detects all major mutation classes
  • Lower uniformity
  • Lower performance in CNV detection
  • Impossible to detect unknown fusion partners
  • Good performance on SNVs / Indels and known fusions
Technology Primer Extension Target Enrichment Hybridization-based capture PCR amplification
Turnaround time Single day 1.5 days Single shift
Panel size Up to 250 Kb Up to Whole Exome and beyond Up to a few megabases

Applications

  • Somatic oncology research
  • RNA fusions (known + novel fusion partners)
  • Hereditary genetics research
  • Hereditary and somatic Oncology research
  • Somatic oncology research
  • Hereditary genetics RNA fusions (Known only)
Secondary analysis support
  • Germline and somatic variants secondary analysis in NAVIFY Mutation Caller
  • Germline and somatic variants secondary analysis with open source bioinformatic tools
  • Germline variants secondary analysis in NAVIFY Mutation Caller
  • Germline and somatic variants secondary analysis with open source bioinformatic tools

 

Various vendor offerings