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Primer Extension-Based Target Enrichment

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Overview

Based on primer extension technology, KAPA HyperPETE technology provides a single workday workflow for target enrichment. The integrated end-to-end offering makes for more efficient and effective processes, enabling you to shift your focus to what matters most. Providing both catalog or custom panels, KAPA HyperPETE Workflow is especially suited for small panel oncology research applications.

Benefits of the KAPA HyperPETE Workflow
  • High uniformity for better sequencing efficiency with less optimization
  • Single day workflow by shorter incubations and reduced hands-on-time
  • Custom design expertise enables access to difficult genomic regions

Related products

Workflow considerations & comparisons

Streamlining every step of the process from pipetting to purchasing, Roche Sample Prep Solutions encompass all the steps required to convert a sample to a sequencing-ready library. From sample collection to library quantification, we offer integrated workflow solutions for different sample types and sequencing applications that are proven, simple and complete.

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  KAPA HyperPETE Workflow KAPA HyperCap Workflow Amplicon-based workflow

Ease of use
  • Easy to use and automate
  • Get your answers faster, without compromising on performance
  • Streamlined 1-day workflow with short incubations and reduced hands-on time
  • Easy to use and automate
  • Streamlined and optimized workflow with low hands-on-time
  • Easy to use and automate
  • Fastest workflow
Performance
  • Highest sequencing efficiency in small panels
  • Detects all major mutation classes
  • High sequencing efficiency
  • Detects all major mutation classes
  • Lower uniformity
  • Lower performance in CNV detection
  • Impossible to detect unknown fusion partners
  • Good performance on SNVs / Indels and known fusions
Technology Primer Extension Target Enrichment Hybridization-based capture PCR amplification
Turnaround time Single day 1.5 days Single shift
Panel size Up to 250 Kb Up to Whole Exome and beyond Up to a few megabases

Applications
  • Somatic oncology research
  • RNA fusions (known + novel fusion partners)
  • Hereditary genetics research
  • Hereditary and somatic Oncology research
  • Somatic oncology research
  • Hereditary genetics RNA fusions (Known only)
Secondary analysis support
  • Germline and somatic variants secondary analysis with open source bioinformatic tools
  • Germline and somatic variants secondary analysis with open source bioinformatic tools

 

Various vendor offerings

 

For Research Use Only. Not for use in diagnostic procedures.

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