Hybridization-Based Target Enrichment

Hybridization-Based Target Enrichment

Overview

Targeted sequencing enables researchers to enrich specific genes, exons and/or other genomic regions to allow sequencing reads to be dedicated to only the regions that are of interest, which results in time and cost savings.1 To enrich specific target regions for next-generation sequencing (NGS) and bioinformatic analysis, hybridization or capture-based target enrichment is a method frequently used. This technique can be applied to single nucleotide polymorphism (SNP) detection, insertion/deletion (indel) detection, copy number variation (CNV) detection and structural variation detection.

 

KAPA HyperCap Target Enrichment Portfolio

Now available is Roche’s new KAPA HyperCap Target Enrichment Portfolio — KAPA HyperExome, KAPA HyperChoice and KAPA HyperExplore probes. Our industry-leading selection of probes will now offer higher performance, higher probe fidelity and in-solution hybridization capture, as well as longer probes with fixed length.

 

Benefits of KAPA HyperCap Target Enrichment Probes

  • Better capture uniformity
  • Lower duplicate rate
  • Higher target coverage
  • Customer design expertise
  • Confident germline SNV/Indel calling with NAVIFY® Mutation Caller
...

KAPA HyperExome Probes

Designed for greater sequencing depth and higher uniformity with low PCR duplicates, KAPA HyperExome Probes are Roche’s next generation of whole exome probe technology. KAPA HyperExome Probes cover the CCDS, RefSeq, Ensembl, GENCODE and ClinVar genomic databases in an efficient ~43 Mb capture target size for improved sequencing efficiency.

Learn more
...

Target Enrichment Custom Probes

Roche introduces its target enrichment custom probes, KAPA HyperChoice and KAPA HyperExplore, for improved target capture NGS. KAPA HyperChoice Probes enable custom human probe design up to 200 Mb, while KAPA HyperExplore Probes are for less well-known or complex custom designs pushed beyond normal boundaries. HyperDesign, a new online tool that uses Roche’s probe design algorithm and selection, allows you to customize target enrichment panels to meet the unique needs of your research.

Learn more
...

Design Share

Developed by Roche scientists in collaboration with world-leading researchers, Design Share panels make it simple for you to access pre-designed target enrichment panels for NGS. With panels available in multiple research areas including hereditary genetics and oncology, neurology, and sudden cardiac death, Design Share panels are fully customizable to fit your research needs and offer a convenient entrance into the NGS target enrichment space.

Learn more
...

KAPA HyperCap SARS-CoV-2 Panel

Sequence the SARS-CoV-2 genome for viral surveillance, evolution and detection of emerging new isolates or strains, by using the KAPA HyperCap SARS-CoV-2 target enrichment panel.

Learn more
...

KAPA HyperCap Fixed Panels

Access relevant content faster and with greater sequencing efficiency. The KAPA HyperCap Fixed Panels are catalog gene panels for target enrichment research applications which are readily available from stock. Based on Roche’s renowned content and probe design expertise, the KAPA HyperCap Fixed Panels offer high sequencing efficiency and answers that matter to your research questions.

Learn more

  1. Kozarewa et al. Curr Protoc Mol Biol., 2015. Overview of target enrichment strategies. 

The KAPA HyperExome probes will replace SeqCap EZ Prime Exome Probes, which will be supported until the end of March 2022.