As next-generation sequencing (NGS) sample preparation entails a multitude of steps and each step is critical for the overall sequencing success and efficiency, all steps need to be optimized in terms of reagents and protocol. Considering the precious nature of NGS samples, an integrated workflow with optimized reagents is necessary for converting the samples into a sequencing-ready library. NGS sample types can differ in terms of their:
Taking into account all these variations, it is important to use library preparation reagents that can work together with all these sample types. High-quality library preparation reagents help in obtaining uniform and improved coverage as well as lower duplication rates. Depending on the end goal (for example, whole exome sequencing), a target enrichment step may also be required for enriching specific regions of interest. Use of high-efficiency target enrichment reagents helps in obtaining uniform and high depth of coverage.
The latest version KAPA HyperCap Workflow combines validated products for both KAPA Library Preparation and KAPA HyperCap Target Enrichment Probes and all accessories and reagents needed for sample preparation into a single workflow. The new KAPA HyperCap Target Enrichment Probes provide high capture efficiency, sequencing uniformity and low duplication and together with the updated KAPA HyperPlus Kits, the workflow delivers better target coverage and higher variant detection confidence. In addition, the workflow includes KAPA Universal Adapter, KAPA Universal UMI Adapter and KAPA UDI primer mixes (up to 384). The KAPA HyperCap Workflow utilizes the renowned probe design and selection algorithm and provides the convenience of simplified ordering and support from a single vendor, and is automation friendly. Sequencing data generated with the KAPA HyperCap Workflow can be analyzed with Roche's cloud-based secondary analysis solution - NAVIFY® Mutation Caller - for accurate detection of germline SNV and indel variants.