Sample preparation workflows for next-generation sequencing (NGS) can be long and tedious, especially when targeted sequencing is needed. First, a sequencing-ready DNA library is prepared through a multi-step process. Each step requires specific types of reagents and accessories. Necessary quality control needs to be performed in between the steps, which also requires compatible reagents. In addition, depending on the end goal, target enrichment may also need to be performed for certain applications. This adds several more reagents and steps that need to be optimized. As each step of the workflow is critical for obtaining reliable sequencing results, it is crucial to have a seamless workflow process.
Typically, reagents for each step in the workflow are procured from various sources and researchers then need to optimize them together in their lab. This can cause variations in sequencing efficiency, which can impact analysis and results. When purchasing reagents from multiple vendors, it can also be hard to determine where any issue may lie, if results are not as anticipated. Roche simplifies this process by providing an integrated solution with high-quality library preparation and high performing target enrichment products optimized together into a single validated workflow.
KAPA HyperCap Workflow is an integrated approach to sample preparation, for applications such as whole exome sequencing, encompassing all the steps required to convert a sample to a sequencing-ready library. It is highly beneficial for your research and for your precious NGS samples to rely on Roche’s Sample Prep Solutions that are proven, simple and complete.