NGS Sample Preparation Reagents

High quality sample prep for sequencing success

Sample preparation for next-generation sequencing (NGS) entails a series of complex steps to allow for construction of DNA and RNA sequencing libraries. Successful sequencing requires high quality library construction of sufficient yield, where the workflow depends on the NGS application. This includes the following stages: input sample QC, DNA or RNA library construction and target enrichment (optional), library amplification and library quantification prior to sequencing.

Because of the number of steps involved and their inherent complexities, each step can potentially introduce errors or biases that could significantly impact the success and reliability of sequencing results. Therefore, the use of high quality reagents and optimized protocols are required to obtain superior sequencing libraries with the lowest bias, and the highest and most uniform coverage.

Roche offers a range of sample preparation reagents and high-quality enzymes engineered through our Directed Evolution Technology for every step of the NGS sample preparation workflow.

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DNA Reagents

Roche offers DNA reagents for library prep, target enrichment and also combines them into an integrated workflow for efficiency and convenience. The KAPA library preparation kits enable high performance library construction, while target-specific solution-based hybridization probes allow for the generation of highly enriched target DNA regions. With the HyperCap Workflow, you can easily integrate all the steps and validated reagents required for DNA library preparation and target enrichment thro ...

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RNA Reagents

Choose KAPA RNA Hyper library preparation kits that combine enzymatic steps and reaction purifications for a streamlined RNA library construction. These workflows offer flexibility with upfront RNA enrichment modules for mRNA capture or rRNA and/or globin transcript depletion designed for high quality library construction, even from low input amounts. The kits provide improved detection of GC-rich and low-abundance transcripts to address the challenges of FFPE-derived material.

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Library Quantification

Maximize sequencing capacity and throughput with optimal and predictable cluster densities using the KAPA Library Quantification Kits. These qPCR-based kits accurately quantify library molecules to offer uniform distribution of reads across all libraries in a pool, optimizing sequencing costs. They provide reliable results across library types, including those from low-quality FFPE samples.

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Library Amplification

Amplify your NGS library using the KAPA HiFi DNA Polymerase, the gold standard for NGS library amplification. This enzyme has been engineered using our directed evolution technology specifically for ultra-high fidelity, high efficiency, low bias and robustness. The KAPA HiFi Kit offers uniform coverage and low duplication rates even with difficult regions.* The KAPA HiFi family also includes KAPA HiFi Uracil+ Kits, which contain a uracil-tolerant variant, KAPA HiFi Uracil+ DNA Polymerase.

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Sample Preparation Accessories

Find all accessories needed for a streamlined NGS workflow through Roche’s portfolio. NGS sample preparation workflow accessories include a portfolio of KAPA Adapters, KAPA Cleanup Beads, capture beads and other reagents and consumables required for DNA and RNA library preparation and target enrichment steps in your NGS workflow.

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NGS Automation

Workflows supporting KAPA DNA and RNA Library Preparation Kits, KAPA Library Quantification Kits and Roche Target Enrichment are validated and optimized for automated, high-throughput NGS library construction.

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*Data on file.