TECHNOLOGY

Liquid Biopsy

Liquid biopsy for ctDNA analysis
Overview

Liquid biopsy offers quick and easy access to tumor samples, as well as the potential for serial sampling. This non-invasive technology makes it feasible to expand oncology research to include cohorts that are unable to undergo a standard biopsy procedure or did not yield adequate tissue samples. With liquid biopsy, researchers can analyze biomarkers circulating in the bloodstream obtained through a conventional blood draw.

Circulating tumor DNA (ctDNA) has gained significance as a biomarker for cancer as they are released into the bloodstream by delocalized tumor cells, thus having the potential to provide a more accurate representation of tumor heterogeneity compared to tissue samples.1

Advantages of liquid biopsy and ctDNA
  • Simple, fast, non-invasive test – easier sample access
  • ctDNA can reveal a cancer’s comprehensive genetic profile1
  • Potentially more accurate representation of tumor heterogeneity and burden1
  • Ablility to perform multiple tests on same subject – potential for longitudinal monitoring
However, there are some key technical challenges with ctDNA:
limited input molecules

There is very little circulating DNA per blood draw2

ultralow detection limit

A very small percentage (as few as 0.01%) of circulating DNA is tumor-derived3

broad coverage

Tumor-specific mutations differ among research subjects4

AVENIO ctDNA Analysis Kits V2, a portfolio of three next-generation sequencing (NGS) liquid biopsy assays, overcome these challenges with proven molecular techniques and proprietary error suppression strategies.5


Learn more about AVENIO ctDNA Analysis Kits V2

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    References

    1. Diaz LA and Bardelli A. Liquid biopsies: genotyping circulating tumor DNA. J Clin Oncol. 2014; 32(6):579-586.
    2. Volik S, Alcaide M, Morin R, Collins C. Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies. Molecular Cancer Research. 2016;14(10):898-908. doi:10.1158/1541-7786.mcr-16-0044.
    3. Francis G, Stein S. Circulating Cell-Free Tumour DNA in the Management of Cancer. International Journal of Molecular Sciences. 2015;16(6):14122-14142. doi:10.3390/ijms160614122.
    4. Salk J, Fox E, Loeb L. Mutational Heterogeneity in Human Cancers: Origin and Consequences. Annual Review of Pathology: Mechanisms of Disease. 2010;5(1):51-75. doi:10.1146/annurev-pathol-121808-102113.
    5. Newman AM, Lovejoy AF, Klass DM, et al. Integrated digital error suppression for improved detection of circulating tumor DNA. Nature Biotechnology. 2016;34(5):547–555. doi:10.1038/nbt.3520.

     

    For Research Use Only. Not for use in diagnostic procedures.