KAPA SYBR Fast One-Step qRT-PCR Kits

For Research Use Only. Not for use in diagnostic procedures.

Overview

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kits contain an M-MuLV reverse transcriptase, an RNase inhibitor, and a novel DNA polymerase engineered through our directed evolution technology.

Benefits of KAPA SYBR FAST One-Step qRT-PCR Kits

Rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination
A convenient qRT-PCR protocol

Product highlights

Increase signal and reaction efficiency

Consistently higher amplification efficiencies *
Higher fluorescence, earlier Cq values and improved reaction efficiencies compared to other options*

View data showing increased signal and reaction efficiency using KAPA SYBR FAST One-Step qRT-PCR Kit compared to a competitor kit.

Figure 1. RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per 20 µL reaction) isolated from human placenta. KAPA SYBR FAST One-Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.

Figure 2. RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per 20 µL reaction) isolated from human placenta. KAPA SYBR FAST One-Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.

Figure 3. RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per 20 µL reaction) isolated from human placenta. KAPA SYBR FAST One-Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.

Amplify across a broad range of target lengths

Robust performance independent of amplicon size

View data showing high qPCR performance using KAPA SYBR FAST One-Step qRT-PCR Kit across varying lengths of RNA targets.

Figure 4. MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 µL reaction. KAPA SYBR FAST One-Step qRT-PCR Kit maintained high performance across all amplicon lengths.

Figure 5. MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 µL reaction. KAPA SYBR FAST One-Step qRT-PCR Kit maintained high performance across all amplicon lengths.

Figure 6. MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 µL reaction. KAPA SYBR FAST One-Step qRT-PCR Kit maintained high performance across all amplicon lengths.

Improve sensitivity and reproducibility

Accurate interrogation across a wide range of RNA template concentrations

View performance data for KAPA SYBR FAST One-Step qRT-PCR Kit showing high and uniform efficiency across varying dilutions of target.

Figure 7. 279 bp fragment amplified in replicates of 6 from a 10-fold dilution series of commercial MS2 bacteriophage RNA (80 pg to 0.8 fg per 20 µL reaction). Reaction efficiency of 95% calculated for all 6 replicates per data.

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For online ordering

*Data on file.

Specifications

Starting Material

RNA
Input Amount

1 pg–1 µg total RNA, 10 fg–100 ng poly(A)RNA, 10^-1x10⁸copies viral RNA

Kits can be stored for up to 6 months at -20˚C.

Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl₂ at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.

Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

Components

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	Kit code	50X KAPA SYBR FAST ROX High	50X KAPA SYBR FAST ROX Low	2X KAPA SYBR FAST qPCR Master Mix (Universal)	2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye)	50X KAPA RT Enzyme Mix	10mM dUTP
Universal	KK4650	X	X	X		X	X
	KK4651
	KK4652
Bio-Rad iCycler	KK4670				X	X	X
	KK4671
	KK4672
Optimized for Roche LightCycler^® 480	KK4680				X	X	X
	KK4681
	KK4682

For purchasing or general questions

For online ordering

Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.

Component

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK4650	07959613001	100 x 20 µL reactions (Universal)	1 mL	Login for pricing
KK4651	07959621001	500 x 20 µL reactions (Universal)	5 mL	Login for pricing
KK4652	07959630001	1000 x 20 µL reactions (Universal)	10 mL	Login for pricing
KK4670	07959702001	100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)	1 mL	Login for pricing
KK4671	07959729001	500 x 20 µL reactions, with fluorescein (Bio-Rad iCycler)	5 mL	Login for pricing
KK4672	07959737001	1000 x 20 µL reactions, with fluorescein (Bio-Rad iCycler)	10 mL	Login for pricing
KK4681	07959761001	500 x 20 µL reactions (optimized for use with Roche LightCycler 480)	5 mL	Login for pricing

For purchasing or general questions

For online ordering

KAPA SYBR Fast One-Step qRT-PCR Kits

Overview

Resources

Product highlights

Specifications

Components

Component