1. Roche Sequencing
  2. QPCR
  3. KAPA SYBR Fast One step

KAPA SYBR FAST One-Step qRT-PCR Kits

Overview

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kits contain an M-MuLV reverse transcriptase, an RNase inhibitor, and a novel DNA polymerase engineered through our directed evolution technology.

Benefits of KAPA SYBR FAST One-Step qRT-PCR Kits

  • Rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination
  • A convenient qRT-PCR protocol

Product Highlights

Increase signal and reaction efficiency

  • Consistently higher amplification efficiencies *
  • Higher fluorescence, earlier Cq  values and improved reaction efficiencies compared to other options*

View data showing increased signal and reaction efficiency using KAPA SYBR FAST One-Step qRT-PCR Kit compared to a competitor kit.

Amplify across a broad range of target lengths

  • Robust performance independent of amplicon size

View data showing high qPCR performance using KAPA SYBR FAST One-Step qRT-PCR Kit across varying lengths of RNA targets.

Improve sensitivity and reproducibility

  • Accurate interrogation across a wide range of RNA template concentrations

View performance data for KAPA SYBR FAST One-Step qRT-PCR Kit showing high and uniform efficiency across varying dilutions of target.

*Data on file.

Featured Resources

Research Use Only. Not for use in diagnostic procedures.
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