Targeted sequencing allows us to focus on specific genes, coding regions, even segments of chromosomes with precision and efficiency. It offers unique insights into specific regions of interest in the genome. Sequencing of targeted regions is cost-effective and aids in deeper analysis of next-generation sequencing (NGS) results. In addition, it affords depth of coverage in sequencing, which helps in avoiding false interpretations of sequencing data.
While predesigned catalog or off-the-shelf probes are readily available for targeting exonic regions, custom probes are often needed to further specific research. Roche offers HyperDesign, a target enrichment probe design software tool that enables researchers to custom design their KAPA Target Enrichment Probes.