The KAPA RNA HyperPrep Kits utilize a novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries. The strand-specific workflow is flexible, supporting library construction from lower-input amounts and degraded samples and is compatible with mRNA capture and ribosomal RNA and/or globin transcript depletion.

Every KAPA RNA HyperPrep Kit includes the same core library construction chemistry with an optional upfront depletion module, making up four workflow solutions: either mRNA capture, rRNA depletion, rRNA and globin depletion or none. Roche Sequencing Solutions provides a complete library preparation solution with KAPA Pure Beads (included) and KAPA Dual-Indexed Adapters (available separately). These kits are designed to be automation-friendly on a majority of commercially available liquid handlers.

Features and Benefits of KAPA RNA HyperPrep Kits

  • Reduced hands-on and overall time through fewer enzymatic and reaction cleanup steps*
  • Strand-specific, sequencing-ready libraries from input RNA in ~4 hours*
  • Single-day library construction, including mRNA capture and rRNA depletion with the KAPA RNA HyperPrep workflow*
  • Robust performance across different sample types and input amounts*
  • High throughput and consistency with an automation-friendly workflow
  • Validated KAPA RNA HyperPrep Kit data analysis solutions provided through the Genialis platform


Learn more about the Roche and Genialis collaboration


Learn about working with degraded inputs and tumor profiling applications.

Presented by Dr. Nancy Nabilsi

Product highlights


Flexible workflow options for a variety of applications

  • Stranded library construction solutions for sequencing of both coding and non-coding transcripts
  • Compatible with a variety of sample types and input amounts, of both high-quality and degraded RNA


Single-day, single-tube library prep

  • Reduce hands-on and overall turnaround time with fewer enzymatic and cleanup steps
  • Produce strand-specific libraries from input RNA in approximately 4 hours
  • Complete the entire workflow, inclusive of upfront RNA enrichment, in a standard work day
  • Achieve high throughput and consistency with automation-friendly workflows


Sequence what matters

  • Effective RNA enrichment (rRNA depletion or mRNA capture), combined with highly efficient library construction, reduces the number of reads associated with unwanted content and PCR duplicates
  • More unique transcripts and genes are detected from a fixed amount of sequencing


Cover your bases with higher uniformity

  • Efficient RNA enrichment and library construction processes results in more even coverage along transcript lengths
  • Library amplification with KAPA HiFi enables better coverage of difficult GC-rich region


Streamlined end-to-end RNA library preparation and analysis workflow

  • By partnering with Genialis to develop an automated, data analysis and visualization solution validated for KAPA RNA HyperPrep Kits, Roche Sequencing Solutions provides customers with an end-to-end RNA workflow that is simple, proven and complete.
  • Allows every processing step to be traced to ensure quality and reproducibility and management of data, projects and collaborations in the cloud. 


*Data on file.