Overview

The KAPA EvoPlus Kits offer improved fragmentation performance, insensitivity to fragmentation inhibitors and reduced sequencing artefacts through our streamlined and fully automatable workflow. Bringing a new and upgraded enzymatic fragmentation and library prep solution to the market will enable researchers to achieve higher confidence with increased sequencing efficiency.

The KAPA EvoPlus Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately). The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods.

 

Features and Benefits of KAPA EvoPlus Kits

  • Drastically reduced sequencing artefacts with insensitivity to inhibitors, fully tunable fragmentation and improved library prep performance
  • Simplified, streamlined and automatable workflow, with combined Fragmentation and A-tailing step in ReadyMix formulations
  • Tubes and plated format increases efficiency and convenience
  • Compatible with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification


Product highlights

 

Enable superior performance and sequencing results

 

  • KAPA EvoPlus Kits provide the benefits of enzymatic fragmentation without drawbacks
  • Improved sequencing metrics, allowing higher confidence in data due to reduction in sequencing artefacts

 

 

Simplified and streamlined workflow

  • Streamlined library prep with combined Fragmentation and A-tailing step
  • ReadyMix formulations, therefore fewer reagents and hands-on-time
  • Tubes and plated format, increased efficiency and convenience
  • Manual and automation friendly
  • Reduces complexity of workflow and provides greater peace of mind

Validated with the KAPA HyperCap Workflow and KAPA HyperPETE Workflow.

NOTE: KAPA HyperPETE Workflow is not available in Japan at Feb 2022. If you have an interest in KAPA HyperPETE Workflow, please contact us.

 

Tunable and trustable fragmentation

  • Library insert sizes adjustable by varying fragmentation time
  • Reproducible insert sizes across a range of GC content and DNA input amounts
  • No impact to fragmentation - insensitive to EDTA (up to 2 mM), as well as numerous other inhibitors

 

Minimal sequence coverage bias

  • Lower sequence bias when compared to other enzymatic fragmentation methods
  • Less bias leads to more uniform sequencing coverage and reduced sequencing costs

*Data on file.
All graphic data is on file, unless otherwise noted.