KAPA EvoPlus Kits for NGS DNA Library Prep

Overview

The KAPA EvoPlus Kits offer improved fragmentation performance, insensitivity to fragmentation inhibitors and reduced sequencing artefacts through our streamlined and fully automatable workflow. Bringing a new and upgraded enzymatic fragmentation and library prep solution to the market will enable researchers to achieve higher confidence with increased sequencing efficiency.

The KAPA EvoPlus Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately). The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods.

Features and Benefits of KAPA EvoPlus Kits

Drastically reduced sequencing artefacts with insensitivity to inhibitors, fully tunable fragmentation and improved library prep performance
Simplified, streamlined and automatable workflow, with combined Fragmentation and A-tailing step in ReadyMix formulations
Tubes and plated format increases efficiency and convenience
Compatible with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification

Product highlights

Enable superior performance and sequencing results

KAPA EvoPlus Kits provide the benefits of enzymatic fragmentation without drawbacks
Improved sequencing metrics, allowing higher confidence in data due to reduction in sequencing artefacts

View comparative data showing negligible sequencing artefacts for both KAPA EvoPlus Kits and KAPA HyperPrep Kits (mechanical fragmentation) compared to other suppliers.

Simplified and streamlined workflow

Streamlined library prep with combined Fragmentation and A-tailing step
ReadyMix formulations, therefore fewer reagents and hands-on-time
Tubes and plated format, increased efficiency and convenience
Manual and automation friendly
Reduces complexity of workflow and provides greater peace of mind

Validated with the KAPA HyperCap Workflow and KAPA HyperPETE Workflow.

NOTE: KAPA HyperPETE Workflow is not available in Japan at Feb 2022. If you have an interest in KAPA HyperPETE Workflow, please contact us.

The KAPA EvoPlus Kit offers a simplified and streamlined workflow to remove the complexities and risk for human error by providing a trusted enzymatic fragmentation solution.

Tunable and trustable fragmentation

Library insert sizes adjustable by varying fragmentation time
Reproducible insert sizes across a range of GC content and DNA input amounts
No impact to fragmentation - insensitive to EDTA (up to 2 mM), as well as numerous other inhibitors

Reproducible enzymatic fragmentation across different input amounts

See performance data showing tunability and consistency in DNA library insert sizes with samples from different input amounts and DNA dilution buffer.

Minimal sequence coverage bias

Lower sequence bias when compared to other enzymatic fragmentation methods
Less bias leads to more uniform sequencing coverage and reduced sequencing costs

The GC distribution for KAPA EvoPlus libraries are equivalent to KAPA HyperPrep libraries which use mechanically sheared DNA (considered industry gold-standard) as the input.

*Data on file.
All graphic data is on file, unless otherwise noted.

Research Use Only. Not for use in diagnostic procedures.
HYPERCAP, KAPA, KAPA EVOPLUS, KAPA HYPERPETE, KAPA HYPERPLUS and KAPA HYPERPREP are trademarks of Roche. All other product names and trademarks are the property of their respective owners.