NGS Sample Preparation Reagents

Determine the probability of sequencing success for low quality samples prior to library construction using qPCR-based technology. The KAPA hgDNA Quantification and QC Kits contain enzymes developed through our directed evolution platform, which provide quantification and sample QC assessment in a single assay.

Roche offers DNA reagents for library prep, target enrichment and also combines them into an integrated workflow for efficiency and convenience. The KAPA library preparation kits enable high performance library construction, while target-specific solution-based hybridization probes allow for the generation of highly enriched target DNA regions. With the HyperCap Workflow, you can easily integrate all the steps and validated reagents required for DNA library preparation and target enrichment thro ...

Choose KAPA RNA Hyper library preparation kits that combine enzymatic steps and reaction purifications for a streamlined RNA library construction. These workflows offer flexibility with upfront RNA enrichment modules for mRNA capture or rRNA and/or globin transcript depletion designed for high quality library construction, even from low input amounts. The kits provide improved detection of GC-rich and low-abundance transcripts to address the challenges of FFPE-derived material.

Maximize sequencing capacity and throughput with optimal and predictable cluster densities using the KAPA Library Quantification Kits. These qPCR-based kits accurately quantify library molecules to offer uniform distribution of reads across all libraries in a pool, optimizing sequencing costs. They provide reliable results across library types, including those from low-quality FFPE samples.

Amplify your NGS library using the KAPA HiFi DNA Polymerase, the gold standard for NGS library amplification. This enzyme has been engineered using our directed evolution technology specifically for ultra-high fidelity, high efficiency, low bias and robustness. The KAPA HiFi Kit offers uniform coverage and low duplication rates even with difficult regions.* The KAPA HiFi family also includes KAPA HiFi Uracil+ Kits, which contain a uracil-tolerant variant, KAPA HiFi Uracil+ DNA Polymerase.

Find all accessories needed for a streamlined NGS workflow through Roche’s portfolio. NGS sample preparation workflow accessories include a portfolio of KAPA Adapters, KAPA Cleanup Beads, capture beads and other reagents and consumables required for DNA and RNA library preparation and target enrichment steps in your NGS workflow.

Workflows supporting KAPA DNA and RNA Library Preparation Kits, KAPA Library Quantification Kits and Roche Target Enrichment are validated and optimized for automated, high-throughput NGS library construction.