Discontinued SeqCap Portfolio

SeqCap EZ Exomes + UTR Probes have been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.



An integrated approach to sample preparation, for applications such as whole exome sequencing, encompassing all the steps required to convert a sample to a sequencing-ready library is beneficial in your research considering the precious nature of NGS samples. Therefore, we offer Roche Sample Prep Solutions, from sample collection to library quantification for different sample types and sequencing applications that are proven, complete and simple.

The SeqCap® EZ Human Exome + UTR Probes enables enrichment of 64 Mb of coding exons and miRNA regions, plus 32 Mb of untranslated regions (UTRs). The kit is based on the same coding exon sources from SeqCap EZ Human Exome Probes v3.0 with expanded coverage of 5'- and 3' UTRs. Information for UTRs was obtained from NCBI Reference Sequence (RefSeq) refGene table from UCSC GRCh37/hg19 March 2012 and Ensembl (GRCh37 v64).

Benefits of the SeqCap EZ Human Exome + UTR Probes

  • A comprehensive exome and UTR enrichment solution
  • Minimized sequencing costs with the superior capture efficiency of our 2.1M overlapping probes
  • Ability to discover more variants compared to all other target enrichment solutions*

Design Files

Design and Annotation files were designed for use with the following products:

  • SeqCap EZ Exome + UTR Kit, 4 Reactions (Catalog No. 06740294001)
  • SeqCap EZ Exome + UTR Kit, 48 Reactions (Catalog No. 06740308001)

Download Design Files 


File Descriptions

BED files are plain text, tab-delimited files which list genomic coordinates. These files may be used to explore design targets and to assess capture performance within the targeted regions. The first 3 columns are chromosome or sequence name, target start (0-based), and target end (1-based). For selected SeqCap EZ designs, the fourth column of primary and capture target BED files shows associated gene annotation for each genomic region.

BED files can be imported into Microsoft Excel as tab-delimited text or visualized using the SignalMap software.

The following files are included in the downloadable zip file:

  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_primary_annotated.bed: This file contains the design primary target (unpadded) in hg19 coordinates and gene annotation in the 4th column.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_capture_annotated.bed: This file contains coordinates showing the probe footprint with no padding in hg19 coordinates.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_UCSCBrowser.bed: This file is for viewing target coordinates in the UCSC Genome Browser.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_coverage_summary: This file describes the primary target size, the percentage of primary target bases directly covered by one or more probes (“Probe_Coverage”), and the percentage of primary target bases with estimated sequencing coverage for typical results (“Estimated_Coverage”).

Demo Data

Demo data is available for evaluation. Please contact Roche representative for your country. 

*Data on file.