Heat-Seq Oncology Panel

Discontinued HEAT-Seq Portfolio

The HEAT-Seq Oncology Panel has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.

Overview

The HEAT-Seq® Oncology Panel is based on amplification-based enrichment employing an advanced version of Molecular Inversion Probe (MIP) technology and combines a fast and easy workflow with powerful SNV detection. The panel contains 5,144 probes, and has a capture target of approximately 245 Kb across 60 commonly targeted oncogenes and is ideal for new variant discovery and validation.

Benefits of Heat-Seq Oncology Panel

Simple workflow with sequencing-ready DNA in a single tube with just two hours of handson time
Detection and removal of PCR duplicates using molecular barcoding and the ability to confidently discern a biological replicate and a process artifact
Maximized variant detection over a wide range of allelic frequencies using molecular barcoding with UIDs without the need for deep sequencing
Minimized sequencing errors due to the stringent removal of false positives
Utilize HSQutils software, which is a separately sourced sequencing read mapper and variant caller for use in the analysis workflow

Heat-Seq Workflow

The final product of the workflow is a sequencing-ready amplicon that contains the target DNA sequence, a molecular barcode, and sample indices.

The entire workflow is completed in 8 hours with < 2 hours of hands-on time using a single tube. The protocol is easy to adopt and is also automation-friendly.

Product Highlights

Target the coding region of 60 oncogenes with one panel

HEAT-Seq Oncology Panel genes

ABL1	EZH2	KDR	PDGFRA
AKT1	FBXW7	KIT	PIK3CA
ALK	FGFR1	KRAS	PTEN
APC	FGFR2	MAP2K1	PTPN11
ATM	FGFR3	MAP2k4	RB1
BRAF	FLT3	MET	RET
BRCA1	GNA11	MLH1	RUNX1
BRCA2	GNAQ	MPL	SMAD4
CDH1	GNAS	MSH2	SMARCB1
CDKN2A	HNF1A	MSH6	SMO
CSF1R	HRAS	NF1	SRC
CTNNB1	IDH1	NF2	STK11
EGFR	IDH2	NOTCH1	TERT
ERBB2	JAK2	NPM1	TP53
ERBB4	JAK3	NRAS	VHL

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Accurate, reliable and uniform performance

HEAT-Seq Oncology Panel performance

The HEAT -Seq Oncology Panel was tested using Coriell HapMap 12878 DNA. Accuracy, reliability and uniformity are demonstrated across many replicates. Each replicate was subsampled to 2 million reads, and yielded a mean coverage across the target of 163X. Uniformity is defined is the percentage of probes giving coverage that is greater than or equal to 20% of the mean.

View performance data showing excellent overall performance and strong SNP detection using the HEAT-Seq Oncology Panel.

Strong detection of known SNPs

HEAT-Seq Oncology Panel Variant Detection.

The HEAT-Seq Oncology Panel was used to measure allele frequency in a sample of formalin-compromised DNA (Quantitative Multiplex Reference Standard, HD-C749, Horizon Discovery) to simulate the effects of FFPE storage and extraction. Detection of variants at known frequencies between 0.9% and 25% is demonstrated with high correlation. Each replicate was tested using 250ng of DNA and data was subsampled to 2 million reads per sample.

View strong detection of variants at known frequencies using the HEAT-Seq Oncology Panel.

Accurate detection of variants due to removal of duplicates and artifacts by UIDs

View data on HEAT-Seq oncology panel specificity and accurate detection of variants at a wide frequency range with zero total false positive rates.*

Chromosome	Coordinate	Expected Variant MAF	Average Variant MAF Detected
chr1	115256529	12.5	6%
chr3	29443695	8.75	9%
chr3	178936091	17.5	15%
chr4	178952085	10	7%
chr7	55241707	24.5	24%
chr7	55259515	0.9	1%
chr7	140453136	2.8	2%
chr7	140453136	10.7	12%
chr12	80409488	15	12%
chr12	123279677	6.25	6%

Design Files

The design and annotation files provide information about genomic regions covered by the capture probes and the genes included in these regions.

These files were designed for use with the following Roche products:

HEAT-Seq Oncology Panel, 24 Reactions (Catalog No. 07757042001)
HEAT-Seq Oncology Panel, 96 Reactions (Catalog No. 07768605001)

Download Design Files

Demo Data

Demo data is available for evaluation. Please contact Roche representative for your country.

*Data on file

Research Use Only. Not for use in diagnostic procedures.
HEAT-SEQ is a trademark of Roche.