HEAT-Seq Choice Designs

Discontinued HEAT-Seq Portfolio

The HEAT-Seq Choice Designs have been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.

Overview

HEAT-Seq® Choice Designs enable you to build custom designs of varying length (between 140 to 27,343 probes) choosing from our database. The probes are empirically tested and balanced, and target thousands of genes associated with human inherited disease.

The Roche Sequencing Solutions HEAT-Seq Probe database, built on our expertise and proprietary technology, makes customization of challenging probe construction (especially for Molecular Inversion Probes (MIPs)) simpler. Selecting probes from our database allows for peace of mind against poor uniformity concerns, dead or poison probes, or creating the probe pools from many individual oligos. Custom HEAT-Seq Choice designs are made using NimbleDesign software.

Benefits of HEAT-Seq Choice Designs

Probes for thousands of genes associated with inherited disease: Probes target both strands of nearly all coding regions enabling straightforward creation of custom designs
Simple and easy custom design creation: Simply input your genes and coordinates and NimbleDesign software will guide you in making a panel that best meets your needs
Small to large designs in one tube: Build custom designs between 140 and 27,343 probes
Read mapping and variant calling: HSQutils software which is a separately sourced analysis tool

Product Highlights

Powerful empirical probe balancing

Robust method for custom probe creation with optimized probe design
Use of empirical performance data to enable quick assembly of custom designs for uniform coverage

View data showing greater uniformity and mean coverage due to adjustments of probe design using HEAT-Seq Choice Design compared to a design without the adjustment.

Higher sequencing coverage compared to unoptimized designs

View data showing significantly better coverage depth over targets than the design with the probes in equimolar concentrations with equal amount of sequencing.

Consistent uniformity even with larger designs

Rapid configuration of uniform designs by leveraging empirical probe performance data
High uniformity regardless of design size

View data showing high uniformity using HEAT-Seq custom designs even with large designs.

Consistent, high quality sequencing metrics

View performance data demonstrating high performing sequencing metrics, such as number of mapped reads, on-target rates, uniform coverage and sensitivity of SNP detection and classification, using HEAT-Seq Choice Probes.

Featured Resources

Research Use Only. Not for use in diagnostic procedures.
HEAT-SEQ and NIMBLEDESIGN are trademarks of Roche.