The HEAT-Seq Choice Designs have been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.
Overview
HEAT-Seq® Choice Designs enable you to build custom designs of varying length (between 140 to 27,343 probes) choosing from our database. The probes are empirically tested and balanced, and target thousands of genes associated with human inherited disease.
The Roche Sequencing Solutions HEAT-Seq Probe database, built on our expertise and proprietary technology, makes customization of challenging probe construction (especially for Molecular Inversion Probes (MIPs)) simpler. Selecting probes from our database allows for peace of mind against poor uniformity concerns, dead or poison probes, or creating the probe pools from many individual oligos. Custom HEAT-Seq Choice designs are made using NimbleDesign software.
Benefits of HEAT-Seq Choice Designs
Probes for thousands of genes associated with inherited disease: Probes target both strands of nearly all coding regions enabling straightforward creation of custom designs
Simple and easy custom design creation: Simply input your genes and coordinates and NimbleDesign software will guide you in making a panel that best meets your needs
Small to large designs in one tube: Build custom designs between 140 and 27,343 probes
Read mapping and variant calling: HSQutils software which is a separately sourced analysis tool
Product Highlights
Powerful empirical probe balancing
Robust method for custom probe creation with optimized probe design
Use of empirical performance data to enable quick assembly of custom designs for uniform coverage