NGS-Based Products for Oncology Research

Overview

The field of molecular oncology has witnessed tremendous progress in the past decade, thanks to advancements in technologies such as next generation sequencing (NGS) and liquid biopsy, a non-invasive sampling method that offers the potential for new applications in oncology research.

Roche Sequencing Solutions offers NGS assay kits and reagents for oncology research applications.

Assays	Products	Benefits
AVENIO Tumor Tissue and ctDNA Analysis Kits* NGS Oncology assays	AVENIO ctDNA Targeted Kit Tumor profiling AVENIO ctDNA Expanded Kit Expanded tumor profiling AVENIO ctDNA Surveillance Kit Longitudinal tumor burden monitoring AVENIO Tumor Tissue Targeted Kit Tumor profiling AVENIO Tumor Tissue Expanded Kit Expanded tumor profiling AVENIO Tumor Tissue Surveillance Kit Baseline for longitudinal monitoring of tumor burden	Tumor tissue and liquid biopsy assays with matching gene panels Flexibility to match the right assay to the right research goal Includes genes in the U.S. National Comprehensive Cancer Network (NCCN) Guidelines¹ All four mutation classes (SNVs, inDels, fusions and CNVs) in a single assay Exceptional analytical performance backed by robust data^2,3 End-to-end workflow from extraction to analysis and reporting in five days

Assays

Products

Benefits

AVENIO Tumor Tissue and ctDNA Analysis Kits*

NGS Oncology assays

AVENIO ctDNA Targeted Kit
Tumor profiling

AVENIO ctDNA Expanded Kit
Expanded tumor profiling

AVENIO ctDNA Surveillance Kit
Longitudinal tumor burden monitoring

AVENIO Tumor Tissue Targeted Kit
Tumor profiling

AVENIO Tumor Tissue Expanded Kit
Expanded tumor profiling

AVENIO Tumor Tissue Surveillance Kit
Baseline for longitudinal monitoring of tumor burden

Tumor tissue and liquid biopsy assays with matching gene panels
Flexibility to match the right assay to the right research goal
Includes genes in the U.S. National Comprehensive Cancer Network (NCCN) Guidelines¹
All four mutation classes (SNVs, inDels, fusions and CNVs) in a single assay
Exceptional analytical performance backed by robust data^2,3
End-to-end workflow from extraction to analysis and reporting in five days

Reagents	Products	Benefits
Sample QC Reagents*	KAPA hgDNA Quantification & QC Kits	Reliable quantification and quality assessment of low-input or FFPE samples to optimize library construction yields and workflows
Target Enrichment Reagents*	KAPA Custom Probes HyperCap Workflow products	Shared Designs contains pre-designed NGS panels Reduced sequencing costs Customization to capture your regions of interest Integrated, streamlined workflow solution
Library Preparation Reagents*	KAPA HyperPrep Kits KAPA HyperPlus Kits	High-quality library construction from FFPE and challenging samples in less than 3 hours Low bias enzymatic fragmentation for construction of high-quality libraries from microbes with extreme genomic GC content
Library Amplification Reagents*	KAPA Library Amplification Kit	Minimal amplification bias for improved sequencing coverage in WGS workflows that require library amplification
Library Quantification Reagents*	KAPA Library Quantification Kits	Accurate quantification of adapter-ligated molecules prior to sample pooling and cluster generation or template preparation for optimal sequencing results

Instruments	Products	Benefits
Automated Tissue Disection	AVENIO Millisect System	Maximize clinical utility of tissue samples by identifying mutations missed with manual dissection and reducing false negative rates^3,4 Increase dissection efficiency and optimize results by capturing only clinically relevant cells³ Improve lab workflow and minimize demands on staff by ensuring consistent sample capture through an automated, digitally assisted process⁵

Instruments

Products

Benefits

Automated Tissue Disection

AVENIO Millisect System

Maximize clinical utility of tissue samples by identifying mutations missed with manual dissection and reducing false negative rates^3,4
Increase dissection efficiency and optimize results by capturing only clinically relevant cells³
Improve lab workflow and minimize demands on staff by ensuring consistent sample capture through an automated, digitally assisted process⁵

Software	Products	Benefits
Decision Support -- Clinical Reporting	NAVIFY Mutation Profiler	Accurate and efficient interpretation of clinical significance of mutations Richly curated and up-to-date knowledge base for thousands of variants, classified in accordance of clinical significance Potential personalized treatment strategies outlined in a concise, professional oncologist report Summarized currently approved or recommended treatments, as well as relevant clinical trials

References

National Comprehensive Cancer Network. October 15, 2016.
Newman AM, Lovejoy AF, Klass DM, et al. Integrated digital error suppression for improved detection of circulating tumor DNA. Nature Biotechnology. 2016;34(5):547–555.
Geiersbach K, Ady N, Welker N, et al. Digitally guided microdissection aids somatic mutation detection in difficult to dissect tumors. Cancer Genetics. 2016;209(1-2):42- 49. 2.
Gustafson H, Theiss N, Hnatyszyn JH. Characterizing heterogeneic tumor profiles with an integrated companion diagnostics approach. Integrative Cancer Science and Therapeutics. 2016;3(4):500-503.
Data on file.

*Research Use Only. Not for use in diagnostic procedures.

AVENIO, KAPA, MILLISECT and NAVIFY are trademarks of Roche. Other product names and trademarks are the property of their respective owners.

Bioinformatics