KAPA Unique Dual-Index (UDI) and KAPA Dual-Indexed (DI) Adapter Kits contain full-length, ready-to-use, QC-tested, dual-indexed Illumina® adapters for ligation-based library construction. Kit specifications are compared and contrasted below.

For additional details related to product shipping, storage and handling, please refer to technical documentation.

  KAPA Unique Dual-Indexed Adapters KAPA Dual-Indexed Adapters
Number of full-length, dual-indexed adapters 96 96
Nature of dual sequencing index Unique Combinational
Number of 8-nt non-reduntant p5 barcodes 96 8
Number of 8-nt non-reduntant p7 barcodes 96 12
Barcodes identical to those used in adapters supplied by illumnia  No* Yes
Designed to mitigate index assignment Yes No
Recommended for instruments with patterned flow cells
(e.g. HiSeq 3000, HiSeq 4000, HiSeq X, Novaseq)
Yes No
Recommended for instruments with non-patterned flow cells
(e.g. MiSeq 3000, NextSeq 4000, HiSeq 2000, HiSeq 2500)
Compatible with KAPA HyperPrep, KAPA HyperPlus
and KAPA RNA HyperPrep Kits
Compatible with methyl-seq applications No
Adapter formulation Ready-to-use, 15 mM solutions; 20mL per adapter**
Kit configuration Hard-shell plate with automation-friendly labelling
and overfills and replacements seats***
KAPA Adapter Dilution Buffer included Yes (25 mL per kit)   
Show More Show Less

* The set of 192 barcode sequences used in KAPA UDI Adapters is exclusive to Roche.

** This is sufficient for four library preps with the KAPA HyperPrep or KAPA HyperPlus Kit if no adapter dilution is required.Generous overfill supports use on automated liquid handling system.

*** KAPA Adapter plates are shipped with peelable seals.Replacement seals for KAPA UDI Adapter Kits are peelable and pierceable whereas replacement seals for KAPA DI Adapters Kits are peelable only.