NGS Automation Support Program

Optimizing NGS sample preparation steps for automation

Roche Sequencing Solutions allows you to unlock the potential of every sample by providing automation scripts and support for our Sample Prep Reagents & Workflows across a wide offering of 3rd-party NGS liquid handling platforms.

Workflows supporting KAPA DNA and RNA Library Preparation Kits, KAPA Library Quantification Kits, KAPA Target Enrichment, and the legacy SeqCap target enrichment products are validated and optimized for automated, high-throughput NGS library construction. Additional workflows for KAPA Target Enrichment are coming soon.

Scripts are currently available for many NGS liquid handling platforms and are continuously being developed to accommodate other Roche Sample Prep Reagents, workflows and liquid handling platforms. In addition, the necessary overage volumes in our 96-reaction kits enable automation-friendly workflows to support your research.

 

NGS Automation Features and Benefits

Features

  • Validated and supported automation scripts and workflows from Roche and 3rd-party vendors 
  • Streamlined and optimized workflows for high performance KAPA DNA or RNA Library Preparation Kits and the HyperCap Workflow using SeqCap target enrichment probes 
  • Necessary overage volumes in 96-reaction kits to enable automation-friendly workflows

Benefits

  • Higher sample throughput
  • Less hand-on time for greater convenience of batch processing up to 96 samples 
  • Greater reproducibility than manual
  • Improved process control 
  • Comparable performance with manual methods

 

Automation Methods Availability

The following tables showcase the vendors with which we have partnered to create automation methods. Search the table below by vendor or by product to find information regarding your specific needs. Please contact your local Roche representative for more information regarding our automation methods and for information on products not listed below. Current target enrichment methods include the recently launched KAPA Target Enrichment as well as the legacy SeqCap products. Additional KAPA Target Enrichment automation methods are in development.

Availability may differ by region. Please contact your local Roche Sales Representative to learn more.

By  Vendor       By Product

PerkinElmer

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • HyperCap v2.3 Workflow
  • SeqCap EZ Probes
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit
  • KAPA TE/HyperCap v3.0 Workflow*

 

*in development

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit
  • HyperCap v2.3 Workflow

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • KAPA RNA HyperPrep Kit with Riboerase
  • KAPA mRNA HyperPrep Kit

Beckman Coulter

Method Source: Roche

  • KAPA HyperPrep Kit
  • KAPA Library Quantification Kit
  • HyperCap v2.3 Workflow
  • KAPA HyperPlus Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with Riboerase (HMR)
  • KAPA mRNA HyperPrep KIt
  • KAPA TE/HyperCap v3.0 Workflow

  Method Source: Beckman Coulter

  • KAPA Library Quantification Kit

Method Source: Beckman Coulter

  • KAPA Library Quantification Kit

Method Source: Beckman Coulter

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • HyperCap v2.3 Workflow*

 

*in development

Method Source: Roche

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • HyperCap v2.3 Workflow
  •  HyperCap v3.0/KAPA TE*
  •  KAPA RNA HyperPrep Kit
  •  KAPA RNA HyperPrep Kit with Riboerase
  •  KAPA mRNA HyperPrep Kit

 

Method Source: Beckman Coulter

  • KAPA Library Quantification Kit
     

*in development

Agilent

Method Source: Agilent

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • SeqCap EZ Probes
  • KAPA Library Quantification Kit

Method Source: Roche

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • HyperCap v2.3 Workflow
  • KAPA TE/HyperCap v3.0 Workflow*
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with Riboerase*

 

Method Source: Agilent

  • SeqCap EZ Probes
  • KAPA mRNA HyperPrep Kit

 

*in development

Eppendorf

Method Source: Eppendorf

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA RNA HyperPrep Kit
  • KAPA mRNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with Riboerase

Hamilton

Method Source: Hamilton

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • HyperCap v2.3 Workflow
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit
  • HyperCap v3.0 Workflow*


*in development

Method Source: Hamilton

  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with Riboerase (HMR)*
  • KAPA mRNA HyperPrep Kit*

 

*in development

Tecan

Method Source: Tecan

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • SeqCap EZ Probes
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit

PrimaDiag

Method Source: PrimaDiag

  • KAPA HyperPlus Kit
  • SeqCap EZ Probes
  • KAPA HyperPrep Kit
  • HyperCap v2.3 Workflow
  • KAPA TE/HyperCap v3.0 Workflow*

 

*in development

Brand

Method Source: Brand

  • KAPA Library Quantification Kit

By  Vendor       By Product

KAPA HyperPrep Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3 NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

 

Method Source: Beckman Coulter

  • Biomek i5 Automated Workstation

Method Source: Agilent

  • Bravo NGS Workstation Option A


Method Source: Roche

  • Bravo NGS Workstation Option B

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (Span-8)

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: PrimaDiag

  • ACSIA NGS (capture)

KAPA HyperPlus Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

 

Method Source: Beckman Coulter

  • Biomek i5 Automated Workstation

Method Source: Agilent

  • Bravo NGS Workstation Option A


Method Source: Roche

  • Bravo NGS Workstation Option B

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (Span-8)

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: PrimaDiag

  • ACSIA NGS (capture)

KAPA Library Quantification Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod


Method Source: Beckman Coulter

  • Biomek NXp
  • Biomek NXp (Span 8)
  • Biomek 4000

Method Source: Roche

  • Bravo NGS Workstation Option A*
  • Bravo NGS Workstation Option B


Method Source: Agilent

  • Bravo NGS Workstation Option A

 

*in development

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR  (Span-8)

Method Source: Brand

  • Liquid Handling Station

KAPA Human Genomic DNA Quantification and QC Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod

Method Source: Roche

  • Bravo NGS Workstation Option B

KAPA TE/HyperCap v3.0 Workflow

Method Source: PrimaDiag

  • ACSIA NGS (capture)

Method Source: PerkinElmer

  • Sciclone G3 NGSx*

 

*in development

Method Source: Roche

  • Biomek FXp and FX, dual pod

Method Source: Roche

  • Bravo NGS Workstation Option B*

 

*in development

Method Source: Hamilton

  • NGS STAR (Span-8)*

 

*in development

HyperCap v2.3 Workflow

Method Source: PrimaDiag

  • ACSIA NGS (capture)

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

Method Source: Roche

  • Bravo NGS Workstation Option B

Method Source: Hamilton

  • NGS STAR (Span-8) 

SeqCap EZ Workflow

Method Source: PerkinElmer

  • Sciclone G3 NGSx

Method Source: Agilent

  • Bravo NGS Workstation Option A
  • Bravo NGS Workstation Option B

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: PrimaDiag

  • ACSIA NGS (capture)

KAPA RNA HyperPrep Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (Span-8)*
  • Vantage*


*in development

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: Roche

  • Bravo NGS Workstation Option B*

 

*in development

KAPA RNA HyperPrep Kit with RiboErase (HMR)

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3 R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

Method Source: Hamilton

  • NGS STAR (Span-8)*
  • Vantage*


*in development

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Roche

  • Bravo NGS Workstation Option B*

 

*in development

KAPA mRNA HyperPrep Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3 R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

Method Source: Agilent

  • Bravo NGS Workstation Option B

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (Span-8)
  • Vantage*


*in development

Method Source: Tecan

  • Freedom Evo NGS Workstation