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NGS Automation Support Program
Optimizing NGS sample preparation steps for automation
Roche Sequencing Solutions allows you to unlock the potential of every sample by providing automation scripts and support for our Sample Prep Reagents & Workflows across a wide offering of 3rd-party NGS liquid handling platforms.
Workflows supporting KAPA DNA and RNA Library Preparation Kits, KAPA Library Quantification Kits and SeqCap target enrichment products are validated and optimized for automated, high-throughput NGS library construction. Workflows for KAPA Target Enrichment are coming soon.
Scripts are currently available for many NGS liquid handling platforms and are continuously being developed to accommodate other Roche Sample Prep Reagents, workflows and liquid handling platforms. In addition, the necessary overage volumes in our 96-reaction kits enable automation-friendly workflows to support your research.
NGS Automation Features and Benefits
Features
- Validated and supported automation scripts and workflows from Roche and 3rd-party vendors
- Streamlined and optimized workflows for high performance KAPA DNA or RNA Library Preparation Kits and the HyperCap Workflow using SeqCap target enrichment probes
- Necessary overage volumes in 96-reaction kits to enable automation-friendly workflows
Benefits
- Higher sample throughput
- Less hand-on time for greater convenience of batch processing up to 96 samples
- Greater reproducibility than manual
- Improved process control
- Comparable performance with manual methods
Automation Methods Availability
The following tables showcase the vendors with which we have partnered to create automation methods. Search the table below by vendor or by product to find information regarding your specific needs. Please contact your local Roche representative for more information regarding our automation methods and for information on products not listed below. Current target enrichment automation methods are for the SeqCap products; KAPA Target Enrichment product automation methods coming soon.
Availability may differ by region. Please contact your local Roche Sales Representative to learn more.
By Vendor By Product
PerkinElmer
Method Source: PerkinElmer
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- KAPA Library Quantification Kit
- KAPA Human Genomic DNA Quantification and QC Kit
- HyperCap Workflow
- SeqCap EZ Probes
- KAPA RNA HyperPrep Kit
- KAPA RNA HyperPrep Kit with RiboErase (HMR)
- KAPA mRNA HyperPrep Kit
Method Source: PerkinElmer
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- KAPA Library Quantification Kit
- KAPA Human Genomic DNA Quantification and QC Kit
- KAPA RNA HyperPrep Kit
- KAPA RNA HyperPrep Kit with RiboErase (HMR)
- KAPA mRNA HyperPrep Kit
- HyperCap Workflow*
*in development
Method Source: PerkinElmer
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- KAPA Library Quantification Kit
- KAPA Human Genomic DNA Quantification and QC Kit
Beckman Coulter
Method Source: Roche
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit*
- KAPA Library Quantification Kit
- KAPA Human Genomic DNA Quantification and QC Kit*
- HyperCap Workflow
- KAPA RNA HyperPrep Kit*
- KAPA RNA HyperPrep Kit with RiboErase (HMR)*
- KAPA mRNA HyperPrep Kit*
*in development
Method Source: Beckman Coulter
- KAPA Library Quantification Kit
Method Source: Roche
- KAPA Library Quantification Kit*
*in development
Method Source: Beckman Coulter
- KAPA HyperPrep Kit*
- KAPA HyperPlus Kit*
*in development
Method Source: Roche
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- HyperCap Workflow*
*in development
Agilent
Method Source: Agilent
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- SeqCap EZ Probes
Method Source: Roche
- KAPA Library Quantification Kit*
*in development
Method Source: Roche
- KAPA HyperPrep Kit*
- KAPA HyperPlus Kit*
- KAPA Library Quantification Kit
- KAPA Human Genomic DNA Quantification and QC Kit
- Library Normalization*
- HyperCap Workflow
Method Source: Agilent
- SeqCap EZ Probes
- KAPA mRNA HyperPrep Kit
Eppendorf
Method Source: Eppendorf
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- KAPA Library Quantification Kit
- KAPA RNA HyperPrep Kit*
- KAPA mRNA HyperPrep Kit*
*in development
Hamilton
Method Source: Hamilton
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- KAPA Library Quantification Kit
- HyperCap Workflow*
- KAPA RNA HyperPrep Kit*
- KAPA RNA HyperPrep Kit with RiboErase (HMR)*
- KAPA mRNA HyperPrep Kit*
*in development
Tecan
Method Source: Tecan
- KAPA HyperPrep Kit
- KAPA HyperPlus Kit
- KAPA Library Quantification Kit*
- SeqCap EZ Probes
- KAPA RNA HyperPrep Kit*
- KAPA RNA HyperPrep Kit with RiboErase (HMR)*
- KAPA mRNA HyperPrep Kit*
*in development
PrimaDiag
Method Source: PrimaDiag
- KAPA HyperPlus Kit
- SeqCap EZ Probes
Brand
Method Source: Brand
- KAPA Library Quantification Kit*
*in development
By Vendor By Product
KAPA HyperPrep Kit
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
- JANUS G3R NGS Express
Method Source: Roche
- Biomek FXp and FX, dual pod
- Biomek i7 Automated Workstation
Method Source: Beckman Coulter
- Biomek i5 Automated Workstation*
*in development
Method Source: Agilent
- Bravo NGS Workstation Option A
Method Source: Roche
- Bravo NGS Workstation Option B
Method Source: Eppendorf
- epMotion 5075 TMX
Method Source: Hamilton
- NGS STAR (8 channel)
Method Source: Tecan
- Freedom Evo NGS Workstation
KAPA HyperPlus Kit
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
- JANUS G3R NGS Express
Method Source: Roche
- Biomek FXp and FX, dual pod
- Biomek i7 Automated Workstation
Method Source: Beckman Coulter
- Biomek i5 Automated Workstation*
*in development
Method Source: Agilent
- Bravo NGS Workstation Option A
Method Source: Roche
- Bravo NGS Workstation Option B
Method Source: Eppendorf
- epMotion 5075 TMX
Method Source: Hamilton
- NGS STAR (8 channel)
Method Source: Tecan
- Freedom Evo NGS Workstation
Method Source: PrimaDiag
- ACSIA NGS (capture)
KAPA Library Quantification Kit
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
- JANUS G3R NGS Express
Method Source: Roche
- Biomek FXp and FX, dual pod
- Biomek 4000*
Method Source: Beckman Coulter
- Biomek NXp
- Biomek NXp (Span 8)
*in development
Method Source: Roche
- Bravo NGS Workstation Option A*
- Bravo NGS Workstation Option B
*in development
Method Source: Eppendorf
- epMotion 5075 TMX
Method Source: Hamilton
- NGS STAR (8 channel)
Method Source: Tecan
- Freedom Evo NGS Workstation*
*in development
Method Source: Brand
- Liquid Handling Station*
*in development
KAPA Human Genomic DNA Quantification and QC Kit
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
- JANUS G3R NGS Express
Method Source: Roche
- Biomek FXp and FX, dual pod*
*in development
Method Source: Roche
- Bravo NGS Workstation Option B*
*in development
Library Normalization
Method Source: Roche
- Bravo NGS Workstation Option B*
*in development
HyperCap Workflow
Method Source: PerkinElmer
- Sciclone G3 NGSx
Method Source: Roche
- Biomek FXp and FX, dual pod
- Biomek i7 Automated Workstation*
*in development
Method Source: Roche
- Bravo NGS Workstation Option B
Method Source: Hamilton
- NGS STAR (8 channel)*
*in development
SeqCap EZ Workflow
Method Source: PerkinElmer
- Sciclone G3 NGSx
Method Source: Agilent
- Bravo NGS Workstation Option A
- Bravo NGS Workstation Option B
Method Source: Tecan
- Freedom Evo NGS Workstation
Method Source: PrimaDiag
- ACSIA NGS (capture)
KAPA RNA HyperPrep Kit
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
Method Source: Roche
- Biomek FXp and FX, dual pod*
*in development
Method Source: Eppendorf
- epMotion 5075 TMX*
*in development
Method Source: Hamilton
- NGS STAR (8 channel)*
*in development
Method Source: Tecan
- Freedom Evo NGS Workstation*
*in development
KAPA RNA HyperPrep Kit with RiboErase (HMR)
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
- JANUS G3 R NGS Express
Method Source: Roche
- Biomek FXp and FX, dual pod*
*in development
Method Source: Hamilton
- NGS STAR (8 channel)*
*in development
Method Source: Tecan
- Freedom Evo NGS Workstation*
*in development
KAPA mRNA HyperPrep Kit
Method Source: PerkinElmer
- Sciclone G3 NGSx
- Zephyr G3 NGS
- JANUS G3 R NGS Express
Method Source: Roche
- Biomek FXp and FX, dual pod*
*in development
Method Source: Agilent
- Bravo NGS Workstation Option B
Method Source: Eppendorf
- epMotion 5075 TMX*
*in development
Method Source: Hamilton
- NGS STAR (8 channel)*
*in development
Method Source: Tecan
- Freedom Evo NGS Workstation*
*in development
NGS Automation Publication
Sample preparation: the heart of NGS. Customer Story, 2014. GENOMICS TECAN JOURNAL
Automation Support Program
Roche Sample Prep Reagents & Workflows