Overview

The KAPA HiFi Uracil+ Kit forms part of the family of KAPA HiFi Kits, where KAPA HiFi HotStart Uracil+ DNA Polymerase is a modified version of KAPA HiFi HotStart DNA Polymerase that is engineered to tolerate uracil residues in bisulfite-treated DNA. Bisulfite treatment of DNA results in the conversion of unmethylated cytosine residues into uracil, while the methylated residues are left unmodified. Enzymes used for library amplification in bisulfite-sequencing workflows must be able to read through uracil residues and tolerate low concentrations of AT-rich DNA. Traditional high-fidelity DNA polymerases are typically not suitable for this type of application as the enzymes stall when a uracil is encountered.

KAPA HiFi HotStart Uracil+ DNA Polymerase has been developed to read through uracil residues and other modified bases, while still retaining the performance benefits of KAPA HiFi HotStart DNA Polymerase, including ultra-high fidelity, high efficiency and low-bias amplification. This results in lower duplication rates and improved coverage of challenging regions.

 

Benefits of KAPA HiFi Uracil+ Kits

  • Higher and more uniform bisulfite-sequencing coverage for templates containing uracil compared to other methods 
  • Higher yields, low duplication rates and fewer wasted sequencing reads
  • Convenience with the consistency of one core enzyme
  • Trusted as shown through over 10 years in the industry as well as thousands of peer-reviewed publications

Product highlights

 

Amplification of uracil-containing template

  • Effective amplification through uracil and other modified residues

 

High-efficiency, low bias amplification

  • Reduced cycles and lower PCR duplicates through high-efficiency amplification of bisulfite-converted libraries.  
  • Low bias amplification results in improved representation of all library fragments, regardless of size. 

 

Higher and more uniform coverage of bisulfite-convert libraries

  • Improved representation of AT-rich and other difficult regions
  • More even coverage leads to higher coverage depth uniformity

 

Efficient target enrichment of bisulfite-treated DNA

  • Enabled by KAPA HiFi HotStart Uracil+ DNA Polymerase to provide high-efficiency, low-bias amplification
  • Higher yields, low duplication rates and fewer wasted sequencing reads with target enrichment workflows