The KAPA HiFi Uracil+ Kit forms part of the family of KAPA HiFi Kits, where KAPA HiFi HotStart Uracil+ DNA Polymerase is a modified version of KAPA HiFi HotStart DNA Polymerase that is engineered to tolerate uracil residues in bisulfite-treated DNA. Bisulfite treatment of DNA results in the conversion of unmethylated cytosine residues into uracil, while the methylated residues are left unmodified. Enzymes used for library amplification in bisulfite-sequencing workflows must be able to read through uracil residues and tolerate low concentrations of AT-rich DNA. Traditional high-fidelity DNA polymerases are typically not suitable for this type of application as the enzymes stall when a uracil is encountered.
KAPA HiFi HotStart Uracil+ DNA Polymerase has been developed to read through uracil residues and other modified bases, while still retaining the performance benefits of KAPA HiFi HotStart DNA Polymerase, including ultra-high fidelity, high efficiency and low-bias amplification. This results in lower duplication rates and improved coverage of challenging regions.