KAPA HyperCap SARS-CoV-2 panel

KAPA SARS-CoV-2 Target Enrichment Panel

KAPA HyperCap SARS-CoV-2 Target Enrichment Panel

Sequence the SARS-CoV-2 genome for viral surveillance, evolution and detection of emerging1 new isolates or strains, by using the KAPA HyperCap SARS-CoV-2 target enrichment Panel. Prepare libraries from input RNA using the robust KAPA RNA HyperPrep Kit and then enrich for viral sequences; this panel targets 100% of the reference SARS-CoV-2 genome (NC_045512) and >99.7% of another 183 publicly available SARS-CoV-2 genomes.

  • Identify multiple variants of SARS-CoV-2 in a single tube workflow*
  • Cover about 97% of the SARS-CoV-2 genome by 1x down to 1000 viral copies, as few as 10 viral copies are enough to retrieve genome reads (in 20 ng or 100 ng universal human reference RNA background with 0.5 million 2 x 75 bp clusters)
  • Report your results faster with hybridization in as short as 1 hour

 

* Project: Analyzing the SARS-COV-2 Genome with Target Enriched RNA-Seq, Wilmington, MA 2021.

1 World Health Organization. (2021). Genomic sequencing of SARS-CoV-2: a guide to implementation for maximum impact on public health, 8 January 2021.
World Health Organization: https://apps.who.int/iris/handle/10665/338480. License: CC BY-NC-SA 3.0 IGO.

Target Enrichment workflow

Identify multiple variants of SARS-CoV-2 in a single reaction

Variants from six RNA controls of SARS-CoV-2 are identified from within a single (contrived) sample. A total of 1,000,000 SARS-CoV-2 genome copies from 6 different viral RNA controls were combined with either 20 ng (blue) or 100 ng (green) of human universal reference RNA and processed in triplicate using KAPA RNA HyperPrep Kit and the KAPA HyperCap SARS-CoV-2 Target Enrichment Panel. Alternate allele frequency analysis shows that all expected variants from the six SARS-CoV-2 controls were identified at close to the expected frequency. Datasets downsampled to 1 million clusters (2 x 75 bp, duplicates retained for this analysis) prior to analysis.

Cover more of the SARS-CoV-2 genome & identify viral sequence from low viral copy numbers

The KAPA HyperCap SARS-CoV-2 Target Enrichment Panel achieves 1X coverage of ~97% of the SARS-CoV-2 genome down to 1000 viral copies and captures genomic sequence from as few as 10 viral copies. Samples containing the indicated number of viral copies in a background of either 20 ng (blue) or 100 ng (green) of human RNA background were processed in triplicate using KAPA RNA HyperPrep Kit and the KAPA HyperCap SARS-CoV-2 Target Enrichment Panel. Datasets were downsampled to 0.5 million clusters 2 x 75 bp prior to analysis.

Save valuable time with hybridization as short as 1 hour

Shortening the hybridization time to 1 hour yields equivalent 1X SARS-CoV-2 genome coverage compared to 16-hour hybridization. Libraries generated from samples containing the indicated number of viral copies in a background of 20 ng of human reference RNA were hybridized to the KAPA HyperCap SARS-CoV-2 Panel for 1 hour or 16 hours and processed through the HyperCap v3 workflow in triplicate. A. The 1-hour hybridization time and 16-hour hybridization time yield similar SARS-CoV-2 genome coverage at 1X for all viral copy levels. B. Samples with higher viral loads also yield similar genome coverage at 20X for the 1-hour and 16-hour hybridization times, with somewhat reduced coverage for lower viral loads. 0.5 million Illumina NextSeq clusters (2x75 bp).