KAPA HyperCap Heredity Panel

KAPA HyperCap Heredity Panel

KAPA HyperCap Heredity Panel offers high performance

The KAPA HyperCap Heredity Panel is a 10 Mb capture target panel covering 3332 genes strongly associated with hereditary disease research, plus the ClinVar pathogenic and likely pathogenic variants content. It provides an optimal balance between relevant content carefully selected by the Roche Sequencing Scientists and sequencing efficiency driven from exceptional uniformity and very low duplication rates. It is readily available from stock and offers consistent quality by NGS probes QC of every lot. Sequencing data generated with the KAPA HyperCap Heredity Panel can be analyzed with Roche's cloud-based secondary analysis solution - the NAVIFY® Mutation Caller - for accurate detection of variants.

Elevate sequencing efficiency

  • Achieve a fine balance of high specificity (80.9% reads on-target), high uniformity (1.42 Fold-80 base penalty) and low PCR duplication (2.2%)
  • Maximize your sequencing efficiency to cover 98.5% of the target bases by 30x and achieve a mean coverage of 92.6x
  • Uncover efficiencies across the sample preparation process with the streamlined and automation-friendly KAPA HyperCap Workflow
  • Simplify your secondary data analysis with confident variant calling, using NAVIFY® Mutation Caller

     
HyperCap Heredity panel key sequencing metrics

Figure 1. Key sequencing metrics with the KAPA HyperCap Heredity Panel. Eight-plex pre-capture multiplexing with the standard KAPA HyperCap Workflow v3 including the KAPA HyperPlus Kit from 100 ng gDNA (16 coriell cell lines in duplicate). Final libraries were sequenced on a NovaSeq™ 6000 system and downsampled to 10 M HQ filtered clusters (2 x 100 bp) prior to analysis.

 

Sequence what matters

  • Rely on Roche’s renowned content and design expertise to focus on relevant up-to-date content
  • Cover more than 85% of the total ClinVar Pathogenic and likely pathogenic content at >30x by targeting those carefully selected 3332 genes in a sequencing efficient 10 Mb capture target
  • Achieve high percentage of bases covered by 30x for the ACMG 73 genes and the ENSEMBL and RefSeq transcripts of the 3332 genes targeted by the KAPA HyperCap Heredity Panel

 

Figure 2. Percent bases covered at 30x and above in key genomic databases using the KAPA HyperCap Heredity Panel. Twelve-plex precapture multiplexing with the standard KAPA HyperCap Workflow v3 and the KAPA HyperPrep Kit from 100 ng gDNA (96 replicates, NA12878). Final libraries were sequenced on a NovaSeq™ 6000 system and downsampled to 10 M high-quality filtered clusters (2 x 100 bp) prior to analysis.

Demo Data

Demo data is available for evaluation. Please contact your local Roche representative