Specifications

Kits can be stored for up to 6 months at -20˚C.

Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl₂ at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.

Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

Specifications

Spec	Description
Starting Material	RNA
Input Amount	1 pg–1 µg total RNA, 10 fg–100 ng poly(A)RNA, 10^-1x10⁸ copies viral RNA

Components

	Kit code	50X KAPA SYBR FAST ROX High	50X KAPA SYBR FAST ROX Low	2X KAPA SYBR FAST qPCR Master Mix (Universal)	2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye)	50X KAPA RT Enzyme Mix	10mM dUTP
Universal	KK4650 KK4651 KK4652	X	X	X		X	X
ABI Prism	KK4660 KK4661 KK4662				X	X	X
Bio-Rad iCycler	KK4670 KK4671 KK4672				X	X	X
Optimized for Roche LightCycler^® 480	KK4680 KK4681 KK4682				X	X	X

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.

KAPA SYBR FAST One-Step qRT-PCR Kits