KAPA3G Plant PCR Kits

Overview

KAPA3G Plant PCR Kits are designed for PCR using purified DNA, DNA prepared by crude extraction methods (crude sample PCR), and for direct PCR (plant material added directly to the PCR reaction). The kit contains KAPA3G DNA Polymerase, a novel third-generation (3G) enzyme that was engineered via our directed evolution technology for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides.

Features and Benefits of the KAPA3G Plant PCR Kit

Fast PCR direct from plant tissues such as leaf discs, seeds and crude plant extracts
Streamlined workflows for transgenic screening
Improved PCR success rates and reproducibility
Efficient amplification of long and difficult targets from all sample types

Product Highlights

Amplification of long targets (up to 5 kb) from crude samples and purified DNA

High yield and specificity with purified DNA and crude samples

View data showing efficient PCR amplification of targets of different lengths from various plant materials using KAPA3G Plant PCR Kits.

Direct PCR from a variety of plant species and tissue types

Direct PCR with leaf disc or seed as template
No need for time-consuming DNA extractions

View data showing PCR amplification of genomic DNA from various plant materials directly using KAPA3G Plant PCR Kits.

Streamlined workflows and improved turnaround times

Perform PCR in half the time compared with wild-type enzymes
Eliminate the need for time-consuming DNA extraction

View data showing significantly reduced reaction time for direct PCR on plant samples using KAPA3G Plant PCR Kits compared to wild-type Taq.

Improved success rates with novel crude sample plant PCR workflow

Use extraction buffer to prepare crude extracts for plant PCR in just 5 minutes
High success rates with even the most challenging sample types

View high-quality PCR data using the novel crude sample plant PCR workflow with KAPA3G Plant PCR Kits.

*Data on file.

For Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.