1. Home
  2. PCR
  3. KAPA2G Robust PCR Kits
  4. Specifications

KAPA2G Robust PCR Kits

Kits can be stored for up to 12 months at -20˚C.

Standard kits include KAPA2G Robust (5 U/μL), KAPA2G Buffer A, KAPA2G Buffer B and KAPA2G GC Buffer (all at 5X, with 1.5 mM MgCl2 at 1X), KAPA Enhancer 1 (5X), and supplemental MgCl2 (25 mM). Kit options include HotStart and dNTP Mix (10 mM each).

ReadyMix formulations (2X) of KAPA2G Robust HotStart DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with or without dye), dNTPs and MgCl2 (at a final concentration of 2 mM).

Specifications
Spec  Description
Starting Material
 Genomic DNA, cDNA, plasmid, lambda
Input Amount
 < 100 ng for genomic DNA, cDNA < 10 ng for less complex DNA
Contents
Components
KAPA2G Robust Standard or HotStart DNA Polymerase (5 U/µL)
5X KAPA2G Buffer A with MgCl2
5X KAPA2G Buffer B with MgCl2
5X KAPA2G GC Buffer with MgCl2
5X KAPA Enhancer 1
MgCl2 (25 mM)
dNTP Mix (10 mM each dNTP; optional)

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.