KAPA2G Robust PCR Kits

Overview

KAPA2G Robust PCR Kits contain a second-generation DNA polymerase engineered to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). The high performance of the DNA polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

Benefits of KAPA2G Robust PCR Kits

High processivity and specific activity, translating to robust PCR performance and high sensitivity
Improved tolerance to common PCR inhibitors

Product Highlights

Efficient amplification of GC- and AT-rich targets

Robust performance across a wide range of GC- and AT-rich templates
Increased PCR success rates

View data showing 100% success* in PCR amplifications of amplicons with high GC content (up to 84%) using KAPA2G Robust PCR Kits and 66% success using wild-type Taq polymerase.

Improved tolerance to common PCR inhibitors

Efficient amplification from crude samples
Higher yield and sensitivity per unit of enzyme

View data showing more efficient amplification of a plasmid DNA sample in the presence of PCR inhibitors such as NaCl, urea, ethanol and SDS using KAPA2G Robust PCR Kits compared to wild type Taq polymerase.

Superior performance in colony PCR compared to wild type Taq

Higher yields and improved consistency of PCR directly from E. coli and yeast cells

View data showing superior PCR amplification of E. coli and yeast colonies using KAPA2G Robust PCR Kits compared to wild-type Taq polymerase.

*Data on file.

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.